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目的比较大鼠肝脏、卵巢和肾上腺胰岛素受体（ｉｎｓｕｌｉｎ ｒｅｃｅｐｔｏｒ， ＩＲ）的结合性质。方法放射配体结合分析法对ＩＲ的数目（用结合容量表示）与亲和力（用饱和常数表示）进行检测。结果大鼠肝脏、卵巢和肾上腺ＩＲ高亲和位点的结合容量分别为７．３５９×１０个／ｍｇ白质、８．０２９×１０个／ｍｇ蛋白质和６．４４０×１０个／ｍｇ蛋白质，饱和常数分别为６．１４７×１０Ｍ－１、１．５２７×１０Ｍ－１、１．０１０×１０Ｍ－１；ＩＲ低亲和位点的结合容量分别为２．４０３×１０个／ｍｇ蛋白质、２．４０３×１０个／ｍｇ蛋白质和２．２５７×１０个／ｍｇ蛋白质，饱和常数分别为２．９２０×１０Ｍ－１、２．００８×１０Ｍ－１和０．４３３×１０Ｍ－１。结论卵巢和肾上腺也存在丰富的ＩＲ，其数量与肝脏的相似，而饱和常数小于肝脏的ＩＲ。提示胰岛素对卵巢和肾上腺可能有重要的调节作用。
Objective To compare the binding properties of rat liver, ovary and adrenal insulin receptor (IR). Methods Radioligand binding assays were used to determine the number of IRs (expressed as binding capacity) and affinity (indicated by saturation constants). Results The binding capacities of IR high affinity sites in liver, ovary and adrenal were 7.359 × 10 cells / mg white matter, 8.029 × 10 cells / mg protein and 6.440 × 10 cells / mg protein, respectively The constants were 6.147 × 10M-1, 1.527 × 10M-1 and 1.010 × 10M-1, respectively. The binding capacity of IR low affinity sites were 2.403 × 10 / mg protein, 403 × 10 cells / mg protein and 2.257 × 10 cells / mg protein with saturation constants of 2.920 × 10M-1, 1.008 × 10M-1 and 0.433 × 10M-1, respectively. Conclusion Ovarian and adrenal glands also have abundant IR, the number of which is similar to that of liver, while the saturation constant is smaller than that of liver. Tip insulin and ovarian adrenal gland may have an important regulatory role.