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目的探讨p73、p63和p53蛋白在喉鳞状细胞癌发生、发展中的作用与意义,为喉鳞癌的临床诊断及治疗提供新的思路,并为基因治疗的探索提供理论依据。方法采用免疫组织化学(S-P法)检测p73、p63、p53蛋白在50例喉鳞状细胞癌、10例癌旁组织及10例正常喉黏膜中的表达,分析3种基因蛋白与喉鳞癌的临床病理特征之间的关系。结果(1)p73表达与局部淋巴结转移成正相关(P<0.01)。p73阳性表达的患者生存率相对较低(P<0.05)。(2)p63表达与临床分期成负相关(P<0.05),与其他临床特征及生存率无关(P>0.05)。(3)p53表达与各临床特征均无关,p53阳性表达的患者生存率相对较低(P<0.05)。结论(1)喉鳞状细胞癌中的p73过表达可能与局部淋巴结转移有关。p73阳性表达可能是患者预后不良的指标。(2)p63过表达的肿瘤恶性度可能相对较低,p63的表达与患者预后无直接联系。(3)p53阳性表达的患者预后较差。(4)提示p53家族可能和喉鳞状细胞癌形成的早期阶段有关。
Objective To explore the role and significance of p73, p63 and p53 in the occurrence and development of laryngeal squamous cell carcinoma, and to provide new ideas for the clinical diagnosis and treatment of laryngeal squamous cell carcinoma and to provide a theoretical basis for the exploration of gene therapy. Methods The expressions of p73, p63 and p53 in 50 cases of laryngeal squamous cell carcinoma, 10 cases of paracancerous tissues and 10 cases of normal laryngeal mucosa were detected by immunohistochemistry (SP method) The relationship between clinicopathological features. Results (1) The expression of p73 was positively correlated with lymph node metastasis (P <0.01). The survival rate of p73-positive patients was relatively low (P <0.05). (2) The expression of p63 was negatively correlated with clinical stage (P <0.05), but not with other clinical features and survival rate (P> 0.05). (3) There was no correlation between p53 expression and clinical features. The survival rate of patients with p53 positive expression was relatively lower (P <0.05). Conclusions (1) The overexpression of p73 in laryngeal squamous cell carcinoma may be related to the local lymph node metastasis. The positive expression of p73 may be an indicator of poor prognosis in patients. (2) The malignant degree of p63 overexpression may be relatively low, and the expression of p63 is not directly related to the prognosis of patients. (3) patients with positive p53 expression have a poor prognosis. (4) Tip p53 family may be related to the early stages of laryngeal squamous cell carcinoma.