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目的:分析非酒精性脂肪性肝病(NAFLD)小鼠肝脏中脂代谢相关基因的表达变化。方法:12周龄成年雄性C57BL/J6小鼠,随机分为对照组及NAFLD组。对照组予以普通饲料,NAFLD组予以高脂饮食喂养8周。分别测定两组小鼠肝功能、血脂及肝脂的变化,苏木精-伊红(HE)及油红O染色后光学显微镜下观察肝脏的形态结构和脂肪变性情况,并用实时荧光定量PCR(qPCR)法检测肝脏脂代谢重要基因的变化。结果:与对照组比较,NAFLD组小鼠的血清总甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及肝脏TG、肝脏TC水平均显著升高(P<0.05)。HE和油红O染色显示NAFLD组小鼠发生了显著的肝脏脂质沉积。此外,NAFLD组小鼠肝脏中脂肪酸转位酶(CD36)表达水平显著高于对照组,而硬脂酰辅酶A去饱和酶1(SCD1)、固醇调控元件结合蛋白(SREBP1c)表达水平在两组小鼠中无统计学差异。结论:高脂饮食诱导的脂肪肝中CD36表达上调,可能参与了NAFLD的发病机制。
Objective: To analyze the changes of lipid metabolism related genes in the liver of non-alcoholic fatty liver disease (NAFLD) mice. Methods: Male C57BL / J6 mice, 12 weeks old, were randomly divided into control group and NAFLD group. The control group was fed the normal diet, while the NAFLD group was fed with high-fat diet for 8 weeks. The changes of hepatic function, blood lipid and hepatic lipid in the two groups of mice were measured respectively. The morphology and fatty degeneration of the liver were observed under light microscope with hematoxylin-eosin (HE) and oil red O staining. Real-time fluorescence quantitative PCR qPCR) was used to detect the changes of hepatic lipid metabolism important genes. Results: Compared with the control group, the levels of total triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and liver TG and liver TC of NAFLD group were significantly increased <0.05). HE and Oil Red O staining showed significant hepatic lipid deposition in NAFLD mice. In addition, the expression level of fatty acid translocase (CD36) in the liver of NAFLD group was significantly higher than that of the control group, while the expression level of SCD1 and SREBP1c was in the two There was no significant difference in group mice. Conclusion: The up-regulation of CD36 in fatty liver induced by high-fat diet may be involved in the pathogenesis of NAFLD.