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开展水果组织的光传输特性检测分析,以猕猴桃组织为例探讨其在632.8 nm波长的光传输特性。创新点:开发基于单积分球的水果组织光传输特性自动检测系统,验证其可靠性并应用于水果组织的分析,并获得猕猴桃组织不同部位在632.8 nm的吸收和散射系数。方法:利用所搭建的单积分球系统,获取三个不同部位(果肉、种子和种子基座)组织的全反射和全透射信息,测量各个组织切片的厚度及折射率。运用逆倍增算法计算得到各组织样本的吸收系数μa和约化散射系数μs’,并根据计算所得结果对猕猴桃不同组织的光传输特性进行比较分析。结论:本实验结果测得猕猴桃不同部位组织的吸收系数和散射系数分别为0.031~0.308 mm-1和0.120~0.946 mm-1,并显示猕猴桃不同部位组织的吸收和散射特性具有显著差异。种子部位受随机分散的种子的影响较大,组织较均匀的果肉和种子基座部位结果证实散射系数大于吸收系数,符合生物组织高散射介质特性。这些结果说明了定量测定组织的光传输特性参数对深入研究光与水果组织相互作用的重要性。
To carry out the detection and analysis of light transmission characteristics of fruit tissue, the light transmission characteristics at 632.8 nm wavelength were studied by taking kiwifruit tissue as an example. Innovative point: The automatic detection system of light transmission characteristics of fruit tissue based on single integrating sphere was developed, the reliability was verified and applied to the analysis of fruit tissue, and the absorption and scattering coefficients of different parts of kiwifruit tissue at 632.8 nm were obtained. Methods: The total reflection and total transmission information of three different tissues (pulp, seed and seed pedestal) were obtained by using the single integrating sphere system. The thickness and refractive index of each tissue section were measured. The absorption coefficient μa and reduced scattering coefficient μs’ of each tissue sample were calculated by the inverse multiplication algorithm. The light transmission characteristics of different tissues of Actinidia chinensis were compared and analyzed. CONCLUSION: The absorption coefficient and scattering coefficient measured in different parts of Kiwifruit are 0.031 ~ 0.308 mm-1 and 0.120 ~ 0.946 mm-1, respectively. The results show that there are significant differences in the absorption and scattering properties between different tissues of Actinidia chinensis. The seeds were more affected by randomly dispersed seeds. The more homogeneous tissue sections of the pulp and seeds confirmed that the scattering coefficient was larger than the absorption coefficient, which accorded with the characteristics of high-scattering media of biological tissues. These results demonstrate the importance of quantitatively determining tissue transmission parameters to further investigate the interaction between light and fruit tissue.