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为了促进堆肥中木质纤维素降解并了解其中微生物群落代谢功能多样性,该文主要采用Biolog微平板法,考察了添加菌剂对牛粪堆肥中木质纤维素降解及微生物群落代谢能力的影响。结果表明:堆肥结束时,菌剂处理的堆肥中纤维素、半纤维素和木质素的降解率分别比对照提高了31.31%、19.57%和14.33%;对平均色度变化分析表明接种菌剂增加了堆肥高温期微生物的细胞代谢能力;Shannon指数、Simpson指数和McIntosh指数分析均显示添加菌剂可以显著提高堆肥前期的微生物多样性;主成分分析结果显示,添加菌剂提高了堆体中微生物对双亲化合物、聚合物、氨基酸和氨基化合物等碳源的代谢能力,从而可促使有机质被更高效降解。
In order to promote the degradation of lignocellulose in compost and understand the functional diversity of microbial communities, Biolog microtiter plate method was used to investigate the effect of adding bacteriocides on the degradation of lignocellulose and the metabolic capacity of microbial communities in cow dung composting. The results showed that the biodegradation rates of cellulose, hemicellulose and lignin were increased by 31.31%, 19.57% and 14.33% compared with the control at the end of compost respectively. The analysis of average color change showed that the inoculation agents increased The microbial metabolism ability of microorganisms at high temperature in composting was analyzed. Shannon’s index, Simpson’s index and McIntosh’s index analysis showed that adding microbial agents could significantly increase the microbial diversity at early composting stage. The principal component analysis showed that adding microbial agents increased the microbial Parents of compounds, polymers, amino acids and amino compounds such as carbon metabolism, which can promote the more efficient degradation of organic matter.