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7d龄纯种伊沙鸡连续皮下注射硫酸庆大霉素10d,用药第4d开始肌肉注射氚标记胸腺嘧啶核苷（3~H-TdR）,于不同时间取出内耳基底乳头进行放射自显影（ARG）观察。发现停药当天组标记细胞多为支持细胞,位于损伤区基底膜上至表层之间,第7d时受损区可见到标记的成熟毛细胞。对照组未见标记的毛细胞和支持细胞,提示庆大霉素中毒后鸡基底乳头中细胞增殖活跃,基底乳头中某些支持细胞作为前体细胞,通过有丝分裂增殖分化为毛细胞。
The 7-day-old pure-breed Isa chicken was injected subcutaneously with gentamicin sulfate for 10 days. The tritium labeled thymidine (3 ~ H-TdR) was injected intramuscularly on the 4th day after drug administration and the autologous endodermis nipple was removed at different times for autoradiography )Observed. It was found that on the day of discontinuation, most of the labeled cells in the group were supporting cells located on the basement membrane in the lesion area and between the surface layers. The labeled mature hair cells could be seen in the damaged area on the 7th day. In the control group, no marked hair cells and supporting cells were observed, suggesting that the cells in the basal papilla of gentamicin poisoned cells were actively proliferated. Some of the supporting cells in the basilar papillae were used as precursor cells and proliferated and differentiated into hair cells through mitosis.