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目的 探讨丹参作用于成纤维细胞 ,减轻创面过度愈合的作用机制。方法 体外培养的成纤维细胞第4～ 6代 ,经不同浓度丹参 (40、80、1 60和 32 0μg/ ml)及不同时间 (1、2、3、4和 5天 )培养后 ,加入丹参 (浓度 60μg/ ml)作用后 ,采用 ELISA法和放射免疫法测定其自分泌转化生长因子 -β1 (TGF-β1 )及表皮生长因子 (EGF)的变化规律。 结果 丹参可抑制成纤维细胞自分泌 TGF- β1 ,且随浓度的增大及作用时间的延长而增强 ,具有统计学意义 (P<0 .0 5) ;但对EGF的自分泌无抑制作用 (P>0 .0 5)。 结论 丹参可选择性抑制 TGF-β1 的自分泌 ,抑制成纤维细胞的增殖及减少细胞外基质的沉积 ,减轻创面过度愈合
Objective To explore the mechanism of Salvia miltiorrhiza effect on fibroblasts and reduce the excessive healing of wounds. Methods Fibroblasts cultured in vitro were cultured in different concentrations of Salvia miltiorrhiza (40, 80, 160, and 300 μg/ml) and cultured at different times (1, 2, 3, 4 and 5 days). (60μg/ml concentration), the changes of autocrine transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) were determined by ELISA and radioimmunoassay. Results Salvia miltiorrhiza could inhibit the autocrine of TGF-β1 by fibroblasts, and it increased with the increase of the concentration and the prolongation of the action time (P<0.05), but had no inhibitory effect on the autocrine of EGF ( P>0 .0 5). Conclusion Salvia miltiorrhiza can selectively inhibit the autocrine of TGF-β1, inhibit the proliferation of fibroblasts and reduce the deposition of extracellular matrix, and reduce the excessive healing of wounds.