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目的观察血管内皮细胞生长因子（ＶＥＧＦ）和血管紧张素Ⅱ（ＡＮＧⅡ）对蛙心包间皮、淋巴孔和心肌生血管的作用，探讨ＶＥＧＦ和ＡＮＧⅡ对心包间皮的通透性、淋巴孔的调控作用和心肌肥厚的影响。方法应用ＶＥＧＦ和ＡＮＧⅡ蛙腹膜腔注射，扫描电镜和光镜观察，计算机图象处理。结果正常蛙心包壁层有一些散在分布的心包淋巴孔和少量血窦状间皮。蛙心肌无血管，其血供仅由心腔内血液直接进入心肌小梁间隙。ＶＥＧＦ组和ＡＮＧⅡ组的心包淋巴孔平均直径分别为１．５０和１．７９ｕｍ，显著高于对照组的０．７２ｕｍ（Ｐ＜０．０１）；淋巴孔的平均分布密度、ＶＥＧＦ组和ＡＮＧⅡ组分别是８．２５／０．１ｍｍ２和１２．８０／０．１ｍｍ２，亦明显高于对照组的３．５７／０．１ｍｍ２（Ｐ＜０．０１）；ＶＥＧＦ组和ＡＮＧⅡ组的心包间皮血窦状结构的面积分别是２４４３．９５ｕｍ２／０．１ｍｍ２和２１２１．７９ｕｍ２／０．１ｍｍ２，明显大于对照组的９９５．０８ｕｍ２／０．１ｍｍ２（Ｐ＜０．０１）；实验组蛙心肌无新生毛细血管形成。结论ＶＥＧＦ和ＡＮＧⅡ可使蛙心包淋巴孔开放数量增多，孔径开大，分布密度增高，具明显调控心包淋巴孔的功能；另外，还能使心包间皮血窦状结构面积增多，进?
Objective To observe the effects of vascular endothelial growth factor (VEGF) and angiotensin Ⅱ (ANGⅡ) on the intermetastatic space, lymphatic stomata and myocardial angiogenesis in the frog center. To investigate the effects of VEGF and ANG Ⅱ on the permeability of the pericardium and the regulation of lymphatic stomata Effect and cardiac hypertrophy. Methods VEGF and ANG Ⅱ frog peritoneal cavity injection, scanning electron microscopy and light microscopy, computer image processing. Results The normal frog pericardial wall has scattered scattered pericardial lymph nodes and a small amount of sinusoids. Frog myocardium without blood vessels, the blood supply only by the intracardiac blood directly into the myocardial trabecular gap. The average diameter of pericardial lymphatic vessels in VEGF group and ANGⅡ group were 1.50 and 1.79um, respectively, which were significantly higher than those in control group (0.72um, P <0.01) 8.25 / 0.1mm2 and 12.80 / 0.1mm2 respectively, which were significantly higher than that of the control group (3.57 / 0.1mm2, P <0.01) The area of sinusoid was 2443.95um2 / 0.1mm2 and 2121.79um2 / 0.1mm2 respectively, which was significantly larger than that of the control group (995.08um2 / 0.1mm2, P <0.01) Angiogenesis. Conclusions VEGF and ANGⅡ can increase the number of open frog pericardial lymphatic stomata, increase the pore size and increase the density of peritoneal cavity, and significantly regulate the function of pericardial lymphatic stomata. In addition, the area of pericardial blood sinusoid is increased,