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目的:建立同时测定橘叶中川陈皮素、橘红素、5-去甲川陈皮素含量的反相高效液相色谱法。方法:采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-乙腈-水(体积比30∶30∶40),流速1.0 mL.min-1,检测波长342 nm;柱温为室温(25℃)。结果:川陈皮素、橘红素、5-去甲川陈皮素线性范围分别为0.239～2.870μg(r=0.9995),0.102～1.224μg(r=0.9995),0.054～0.643μg(r=0.9995);平均回收率(n=6)分别为98.06%,98.02%,97.61%,RSD分别为2.6%,1.9%,2.0%。结论:本法快速、简便、准确,重复性好,可用于橘叶川陈皮素、橘红素、5-去甲川陈皮素的定量分析和质量控制。
OBJECTIVE: To establish a reversed-phase high performance liquid chromatography (HPLC) method for the simultaneous determination of nobiletin, citrinin, and 5-desmethyltransferase in mandarin orange leaves. METHODS: The mobile phase was methanol-acetonitrile-water (30:30:40 by volume) with a flow rate of 1.0 mL · min-1 on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) For room temperature (25 ℃). Results: The linear ranges of nobiletin, uridin and 5-desmethyltransplatin were 0.239 ~ 2.870μg (r = 0.9995), 0.102 ~ 1.224μg (r = 0.9995) and 0.054 ~ 0.643μg The recoveries (n = 6) were 98.06%, 98.02% and 97.61%, respectively, with RSDs of 2.6%, 1.9% and 2.0%, respectively. Conclusion: The method is rapid, simple, accurate and reproducible. It can be used for the quantitative analysis and quality control of tangerin, orange and 5 - desmethyltransferase.