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目的:建立大鼠急性心肌缺血/再灌注模型的改良方法与传统方法并进行比较。方法:将60只SD大鼠随机分为两组,每组30只;一组采用改良方法建立模型,即气体麻醉,以气体麻醉面罩通气,短时间开胸;另一组采用传统方法建立模型,即腹腔麻醉,气管插管,长时间开胸。比较两种方法的手术时间、手术成功率、术中血氧饱和度、术后存活率、心肌梗死面积,以及术后大鼠的生活状况。结果:与传统方法组相比,改良方法组大鼠术中存活率升高[(93.3±4.6)%vs.(72.4±8.4)%,P<0.05]、开胸时间缩短为[(6.5±2.0)minvs.(44.9±2.7)min,P<0.01],开胸前后平均血氧饱和度增加[(96.3±0.8)%vs.(90.9±2.1)%,P<0.05],术后存活率提高(82.83%vs.58.3%,P<0.01)。另外改良方法组大鼠术后恢复正常行为活动需要的时间较短,但两组大鼠心肌梗死的面积[(33.5±2.2)%vs.(35.0±3.0)%]无统计学意义。结论:改良方法具有简单、高效及可独立操作的优点,能有效提高建立大鼠急性心肌缺血/再灌注模型的成功率。
Objective: To establish an improved and traditional method of acute myocardial ischemia / reperfusion model in rats and to compare them. Methods: Sixty Sprague-Dawley rats were randomly divided into two groups (30 rats in each group). One group was established by modified method, namely gas anesthesia, gas anesthesia mask ventilation and thoracotomy for a short time. The other group was established by traditional method , That is, abdominal anesthesia, endotracheal intubation, prolonged thoracotomy. The operation time, operation success rate, intraoperative oxygen saturation, postoperative survival rate, myocardial infarction area, and postoperative survival status of the two methods were compared. Results: Compared with the traditional method group, the survival rate of the improved group was significantly higher than that of the traditional method group [(93.3 ± 4.6)% vs (72.4 ± 8.4)%, P <0.05] (96.9 ± 0.8)% vs (90.9 ± 2.1)%, P <0.05]. The postoperative survival rate was significantly higher than that of the control group (2.0 vs 2.0 min min, 44.9 ± 2.7 min, P 0.01) Increase (82.83% vs.58.3%, P <0.01). In addition, the modified method group had a shorter time to resume normal behavior after operation, but the area of myocardial infarction in both groups [(33.5 ± 2.2)% vs (35.0 ± 3.0)%] was not statistically significant. Conclusion: The improved method has the advantages of simple, efficient and independent operation, which can effectively improve the success rate of establishing acute myocardial ischemia / reperfusion model in rats.