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利用具有多态性的31对SSR引物,对国内外191份不结球白菜的遗传多样性及亲缘关系进行了分析。引物平均多态性信息含量(PIC)为0.34;供试不结球白菜品种(系)的平均期望杂合度(He)为0.41,Shannon’s信息指数(I)为0.65;遗传分化系数(Gst)为0.20,说明80%的遗传多样性来自品种(系)之间的差异。不同生态区域的遗传多样性具有差异:国内高于国外,且江淮流域最高。利用UPGMA法作图,将供试的191份品种(系)聚为六大类,同时Structure种质遗传结构分组结果与UPGMA法聚类结果基本吻合,两种分析方法均说明了类群划分与地理分布有一定相关性。研究结果表明不结球白菜品种(系)具有丰富的遗传多样性,大部分品种(系)的亲缘关系聚类是按照地理来源及种质遗传背景进行,本研究为不结球白菜育种研究提供了参考。
The genetic diversity and genetic relationship of 191 non-heading Chinese cabbages at home and abroad were analyzed using 31 pairs of SSR primers with polymorphism. The average polymorphism information content (PIC) of primer was 0.34. The average expected heterozygosity (He) and Shannon’s information index (I) of non-heading Chinese cabbage varieties (lines) were 0.41 and 0.65 respectively. The genetic differentiation coefficient 0.20, indicating that 80% of the genetic diversity comes from the differences among varieties (lines). Differences in genetic diversity among different ecoregions: higher in China than abroad and highest in the Yangtze-Huaihe River basin. UPGMA method was used to map the 191 cultivars (lines) collected in the experiment into six major categories, and the result of genetic structure of Structure genetic structure was basically consistent with that of UPGMA method. Both analysis methods showed that the classification and geographical Distribution has some relevance. The results showed that the genetic diversity of non-heading Chinese cabbage varieties (lines) was rich. The genetic relationship of most cultivars (lines) was based on geographical origin and germplasm genetic background. This study provided the research results for non-heading Chinese cabbage breeding For reference.