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目的:研究11-羰基-β-乙酰乳香酸(AKBA)的抗氧化作用及胃保护效果。方法:30只SD大鼠随机分为5组:正常组(生理盐水5 mL/kg),模型组(吲哚美辛48 mg/kg),西咪替丁组(100 mg/kg),低剂量AKBA组(100 mg/kg),高剂量AKBA组(200mg/kg)。预给药1小时后,用吲哚美辛(48 mg/kg)灌胃造模,评价溃疡指数(UI)、胃内容物酸度(p H)、胃壁黏液量(GWM)、前列腺素E2(PGE-2)及一氧化氮(NO)的含量,并检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性,丙二醛(MDA)含量及核转录相关因子2(Nrf2)和血红素加氧酶1(HO-1)的表达量。结果:(1)与造模组相比,AKBA高剂量组(P<0.05)与西咪替丁组(P<0.001)显著降低了吲哚美辛诱导的胃粘膜损伤,AKBA低剂量组的差异无统计学意义;(2)AKBA与西咪替丁均显著升高胃内容物p H(P<0.001)(AKBA低剂量组P<0.05),增加GWM含量(P<0.001);(3)与造模组相比,AKBA及西咪替丁组均显著增高PGE-2及NO含量(P<0.001);(4)与造模组相比,AKBA及西咪替丁组均显著增高SOD活性(P<0.001)(AKBA低剂量组P<0.05)及CAT活性(P<0.001)并降低了MDA含量(P<0.001);(5)与造模组相比,AKBA高剂量组及西咪替丁组促进Nrf2及HO-1表达明显增高(P<0.05)。结论:AKBA有较好的胃保护效果,同时可以通过升高GWM、降低胃内容物p H,升高NO含量,阻止PGE-2的降低,同时恢复SOD、CAT的活性,降低MDA含量,促进Nrf2及HO-1的表达发挥胃黏膜保护的作用。
Objective: To study the antioxidative effect and gastric protective effect of 11-carbonyl-β-acetyl boswellic acid (AKBA). Methods: Thirty SD rats were randomly divided into 5 groups: normal group (5 mL / kg saline), model group (indomethacin 48 mg / kg), cimetidine group (100 mg / kg) The dose of AKBA (100 mg / kg) and the high dose of AKBA (200 mg / kg). One hour after premedication, the rats were anesthetized with indometacin (48 mg / kg) for ulceration index (UI), gastric contents (p H), gastric mucus (GWM), prostaglandin E2 (PGE-2) and nitric oxide (NO) were measured. The activities of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and nuclear transcription factor 2 (Nrf2 ) And heme oxygenase 1 (HO-1). Results: (1) Indomethacin-induced gastric mucosal lesion was significantly decreased in AKBA high-dose group (P <0.05) and cimetidine group (P <0.001) (2) Both AKBA and cimetidine increased gastric contents p H significantly (P <0.001) (AKBA low dose group, P <0.05), increased GWM content (P <0.001); (3) Compared with the model group, AKBA and cimetidine significantly increased the content of PGE-2 and NO (P <0.001). (4) Compared with model group, AKBA and cimetidine group were significantly increased (P <0.001), AKBA low dose group (P <0.05) and CAT activity (P <0.001) and decreased MDA content (P <0.001). (5) Compared with model group, AKBA high dose group and Cimetidine group promoted the expression of Nrf2 and HO-1 significantly increased (P <0.05). Conclusions: AKBA has good gastric protective effect, meanwhile it can increase GWM, decrease p H of gastric contents, increase NO content, prevent the decrease of PGE-2, restore the activities of SOD and CAT, decrease the content of MDA, Nrf2 and HO-1 expression play a role in gastric mucosal protection.