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Soybean Dongnong 47 was subjected to the experiments of increasing UV-B radiation and water stress on soybean yield components in different growth periods. The results showed that 100-seed weight greatly increased during the early stage of pod filling in the treatment of weak UV-B radiation, seed number per plant as well as seed weight per plant and Dongnong47 yield also increased, while the yield and yield components of Dongnong47 during the blossom to mature period were negatively affected in the treatment of intensive UV-B radiation. 100-seed weight of Dongnong47 all increased in the double factor treatments of UV-B radiation and water stress, with the drought intensified, seed number per plant, seed weight per plant and yield of Dongnong47 decreased, the change of 100-seed weight were various and the antagonistic action of UV-B radiation and water stress were related with their intensity.