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目的建立小鼠膜性肾病模型。方法取6周龄健康雄性BALB/c小鼠20只,分为对照组和模型组。模型组小鼠皮下注射0.2 mg阳离子牛血清白蛋白(C-BSA)和等体积的完全弗氏佐剂,对照组皮下注射生理盐水和完全弗氏佐剂。2周后,模型组小鼠尾静脉注射阳离子牛血清白蛋白,每周3次,隔日注射,对照组使用生理盐水代替。模型的鉴定则采用血液生化检查和病理检查(PASM染色、IgG免疫荧光染色、透射电镜)等方法。结果 8周后12只模型组小鼠出现高蛋白尿、低蛋白血症和高胆固醇血症;PASM染色显示出类似于早期膜性肾病,IgG免疫荧光染色显示出沿毛细血管壁呈颗粒状物沉积,透射电镜下有足突的广泛融合。结论 C-BSA尾静脉注射小鼠可成功构建小鼠膜性肾病模型。
Objective To establish mouse membranous nephropathy model. Methods Twenty healthy male BALB / c mice aged 6 weeks were divided into control group and model group. Mice in the model group were injected subcutaneously with 0.2 mg of cationic bovine serum albumin (C-BSA) and an equal volume of complete Freund’s adjuvant. The control group was subcutaneously injected with normal saline and complete Freund’s adjuvant. Two weeks later, model mice were injected intravenously with cationic bovine serum albumin three times a week, and injected every other day. The control group was replaced by saline. Identification of the model using blood biochemical tests and pathological examination (PASM staining, IgG immunofluorescence staining, transmission electron microscopy) and other methods. Results After 12 weeks, 12 mice in model group showed high proteinuria, hypoalbuminemia and hypercholesterolemia. PASM staining showed similar to early membranous nephropathy. IgG immunofluorescence staining showed that granular cells along the capillary wall Deposition, transmission electron microscopy with a wide range of foot fusion. Conclusion The mice model of membranous nephropathy can be successfully constructed by injecting tail vein of C-BSA into mice.