论文部分内容阅读
目的从人卵泡液代谢组学及Ca~(2+)浓度,探讨二至天癸颗粒提高卵细胞质量的机制。探讨体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)周期人卵泡液代谢组学及钙离子(Ca~(2+))浓度、胆碱脂酶(ChE)、肌酐(Cr)对卵细胞质量及妊娠结局的影响。方法57例IVF-ET患者随机分为试验组(二至天癸颗粒加IVF-ET常规用药组,27例)和对照组(六味地黄颗粒合IVF-ET常规用药组,30例)。观察患者取卵日肾阴虚证候较垂体降调节后的改善情况,比较两组患者卵泡液Ca~(2+)浓度、胆碱脂酶、肌酐的差异及两组卵泡液代谢组学的变化,取卵数、受精率、卵裂率、优质胚胎率和妊娠率的差异。结果试验组治疗后肾阴虚证候积分为8.30±1.46,受精率0.82±0.09,卵裂率0.97±0.07,优质胚胎率0.51±0.18,对照组分别为9.16±1.15,0.74±0.18,0.91±0.10,0.41±0.09,试验组优于对照组(P<0.05)。主成分分析法分析两组卵泡液代谢组学,试验组主要分布在Ⅰ区;对照组主要分布在Ⅱ区。两组卵泡液代谢组学有明显差异。妊娠组卵泡液主要分布在Ⅰ区。试验组Ca~(2+)浓度显著高于对照组(P<0.05)。ChE、Cr高于对照组,但差异无统计学意义。结论在调节卵泡液代谢组学和Ca~(2+)浓度,提高卵细胞、胚胎质量,提高IVF-ET妊娠率方面,二至天癸颗粒优于六味地黄颗粒。
OBJECTIVE: To investigate the mechanism of Erzhi Tiangui granule in improving egg quality from human follicular fluid metabonomics and Ca ~ (2+) concentration. To investigate the metabolites of human follicular fluid in vitro fertilization and embryo transfer (IVF-ET), the levels of Ca2 +, cholinesterase (ChE), creatinine (Cr) Effect on egg mass and pregnancy outcome. Methods 57 patients with IVF-ET were randomly divided into experimental group (n = 27) and control group (n = 20). To observe the improvement of ovarian and kidney-yin deficiency syndrome in ovariectomized rats compared with the hypophyseal regulation, and to compare the difference of follicular Ca 2+ concentration, cholinesterase and creatinine between the two groups and the difference of follicular fluid metabonomics between the two groups Changes, number of oocytes, fertilization rate, cleavage rate, the difference between high quality embryo and pregnancy rate. Results After treatment, the score of syndrome of kidney-yin deficiency in experimental group was 8.30 ± 1.46, the fertilization rate was 0.82 ± 0.09, the cleavage rate was 0.97 ± 0.07, the high quality embryo rate was 0.51 ± 0.18, and the control group was 9.16 ± 1.15,0.74 ± 0.18,0.91 ± 0.10, 0.41 ± 0.09, the experimental group was better than the control group (P <0.05). The principal component analysis of follicular fluid metabolomics analysis of two groups, the experimental group mainly in Ⅰ area; the control group mainly in Ⅱ area. Two groups of follicular fluid metabolomics have significant differences. Pregnancy group follicular fluid mainly in Ⅰ area. The concentration of Ca ~ (2+) in the experimental group was significantly higher than that in the control group (P <0.05). ChE, Cr higher than the control group, but the difference was not statistically significant. Conclusions Erzhi tian decoction is superior to Liu Wei Di Huang granules in regulating follicular fluid metabonomics and Ca 2+ concentration, improving egg mass and embryo quality, and improving pregnancy rate of IVF-ET.