四、实验技术高速液相色谱在实际应用中,各种实验操作技术诸如分离方法的选择,层析柱填充剂的处理,镀渍、装柱,冲洗液的纯化,选择等等是直接影响分离效果和速度的关键因素。如前所述,色谱分离的总分离度Rs与层析柱的理论塔板数N(或理论板高H),容量因素k′和相对保留比α有关。其中要达到高的板数,除选用前述的各种填充剂外,还必须采用正确的装柱方法使柱内得到均一致密的铺层。 Fourth, the experimental techniques High-speed liquid chromatography in practical applications, a variety of experimental techniques such as the separation method of choice, the column packing filler treatment, plating, packing, washing fluid purification, selection and so on are directly affect the separation The key factor of effect and speed. As mentioned above, the overall resolution Rs of the chromatographic separation is related to the theoretical plate number N (or theoretical plate height H), the capacity factor k ’, and the relative retention ratio α of the column. Which to achieve a high number of plates, in addition to the above selection of various fillers, but also must be the correct method of packing column to get a uniform dense ply.