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应用Ｗｉｓｔａｒ大鼠饲喂甲氨基偶氮苯（３′－ＭｅＤＡＢ）造成实验性肝癌模型，与此同时饲喂刺五加多糖动态观察了癌变过程中，肝脏ｒ－谷氨酰转肽酶（ｒ－ＧＴ）活性，肝脏Ｎ－乙酰氨基葡萄糖转移酶Ⅲ（ＧｎＴⅢ）和ｒ－ＧＴ免疫组化定位、肝脏病理组织等和脏器系数等改变，结果显示：（１）ｒ－ＧＴ活性于实验第４天即明显升高（２．３倍），至实验结束时可达对照组的４０倍，ＧｎＴⅢ和ｒ－ＧＴ免疫组化定位，在癌变前期增生结节的胞浆中已有明显表达，随病程进展其改变的强度均与病理组织学检测结果相吻合，表明ＧｎＴⅢ和ｒ－ＧＴ在反映肝细胞癌变方面，确实是一个早期应用价值的指标。（２）刺五加多糖对实验性肝癌的形成似有一定的减缓作用。
Wistar rats were fed with methylaminoazobenzene (3′-MeDAB) to induce experimental hepatoma model, and at the same time, Acanthopanax senticosus polysaccharides were dynamically observed during the process of carcinogenesis. Liver r-glutamyl transpeptidase (r) -GT activity, immunohistochemical localization of hepatic N-acetylglucosaminyltransferase III (GnTIII) and r-GT, histopathological changes in liver tissue, and organ coefficient, etc. The results showed that: (1) r-GT activity was observed in the experiment. After 4 days, it was significantly increased (2.3 times), and by the end of the experiment, it was 40 times that of the control group. GnTIII and r-GT were immunohistochemically localized and were clearly expressed in the cytoplasm of proliferating nodules in the precancerous stage. The intensity of the changes along with the progression of the disease was consistent with the results of histopathological examination, indicating that GnTIII and r-GT are indeed an indicator of early application value in reflecting hepatocellular carcinogenesis. (2) Acanthopanax Senticosus polysaccharide seems to have a certain mitigation effect on the formation of experimental liver cancer.