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目的:研究洋参果总皂苷对培养心肌细胞缺氧及缺氧/复氧损伤的保护作用。方法:对培养的心肌细胞建立缺氧及缺氧/复氧损伤型,实验分9组:正常细胞培养组、缺氧/复氧损伤模型组、缺氧/复氧损伤+洋参果(100、50、25、12.5、6.25、3.125、1.5625μg/ml)组。分别观察单纯缺氧和缺氧/复氧后心肌细胞搏动次数及心肌细胞形态。结果:洋参果总皂苷12.5μg/ml、25μg/ml、50μg/ml浓度,增强缺氧后心肌细胞搏动次数;从形态学观察:单纯缺氧及缺氧/复氧后,洋参果总皂苷在3.125μg/ml~50μg/ml浓度范围,给药组比阴性对照组细胞形态完整。结论:洋参果总皂苷对心肌细胞缺氧及缺氧/复氧损伤有保护作用。
Objective: To study the protective effects of total saponins from cucurbitacin on hypoxia and hypoxia/reoxygenation injury in cultured cardiomyocytes. METHODS: Hypoxia and hypoxia/reoxygenation injury models were established in cultured cardiomyocytes. The experiment was divided into 9 groups: normal cell culture group, hypoxia/reoxygenation injury model group, hypoxia/reoxygenation injury+ginseng fruit (100 , 50, 25, 12.5, 6.25, 3.125, 1.5625 μg/ml) groups. The number of myocardial beats and the morphology of myocardial cells were observed after hypoxia and hypoxia/reoxygenation. Results: The concentration of total saponins in ginseng fruit was 12.5μg/ml, 25μg/ml, and 50μg/ml, and the number of beatings of myocardial cells after hypoxia was increased. From the morphological observation: After anaerobic and hypoxia/reoxygenation, the total ginseng fruit Saponin concentration range from 3.125μg/ml to 50μg/ml, and the morphology of the cells in the administration group was more complete than that in the negative control group. Conclusion: The total saponins of ginseng fruit have protective effects on myocardial hypoxia and hypoxia/reoxygenation injury.