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作者用冰冻蚀刻法研究豚鼠内耳壶腹嵴暗细胞和黑素细胞的超微结构。将15只豚鼠内淋巴囊和内淋巴导管阻塞,1～18个月后分别处死,死后1～3分钟内打开迷路,用多聚甲醛固定2小时,经二甲胂酸盐缓冲液冲洗后放入到甘油、二甲胂酸盐缓冲液中迅速冷冻,蚀刻后喷铂和碳,再用电镜观察。该项实验证实了在超薄切片中观察到的结果,即暗细胞基底部有细小的突起,扩大了基底部的面积。显示了顶部细胞膜上有较多的颗粒,这种颗粒是一种膜内蛋白,可能是离子通道和酶的复合物。偶尔在暗细胞胞膜上还可见到一些火山口样的凹陷,这代表了微小胞饮互相融合处。黑素细胞
The authors used freeze-etching method to study the ultrastructure of dark cells and melanocytes in guinea pig’s inner ear ampulla. 15 guinea pig endolymphatic sac and endolymphatic duct obstruction, were 1 to 18 months after sacrifice, 1 to 3 minutes after death, open the lost, fixed with paraformaldehyde for 2 hours, washed by cacodylate buffer Into glycerol, cacodylate buffer rapid freezing, platinum and carbon after spraying, and then observed by electron microscopy. This experiment confirms the result observed in ultrathin sections, that is, there are fine protrusions on the base of the dark cells, which enlarges the area of the base. Shows that there are more particles on the top cell membrane, which is an intramembrane protein that may be a complex of ion channels and enzymes. Occasionally in the dark cell membrane can also see some crater-like depression, which represents the micro-cell drink each other at the merging. Melanocytes