论文部分内容阅读
目的 研究转染Ｂ７－１基因后的小鼠宫颈癌Ｕ１４ 细胞在体内的免疫原性变化。方法 （１）分别用病毒感染法和电穿孔法将ＬＮＳｍ Ｂ７ 和ｐＣＥＰｍ Ｂ７ 导入Ｕ１４细胞，用流式细胞仪检测阳性混合克隆细胞 Ｕ１４／ＬＮＳｍ Ｂ７ 和 Ｕ１４／ｐＣＥＰｍ Ｂ７ 的Ｂ７－１ 分子表达率；（２）取不同数量的Ｕ１４、Ｕ１４／ＬＮＳｍ Ｂ７ 和Ｕ１４／ｐＣＥＰｍ Ｂ７ 细胞分别皮下接种昆明种小鼠，４周后观察各组小鼠肿瘤大小和病理结果；（３）取１×１０７ 数量的Ｕ１４、Ｕ１４／ＬＮＳｍ Ｂ７ 和Ｕ１４／ｐＣＥＰｍ Ｂ７ 减活细胞疫苗分别免疫小鼠２ 次后，分离脾脏Ｔ 淋巴细胞并测定细胞毒Ｔ淋巴细胞（Ｃｙｏｔｏｔｏｘｉｃ Ｔ ｌｙｍ ｐｈｏｃｙｔｅｓ，ＣＴＬｓ）对Ｕ１４ 细胞的体外杀伤活性。结果 （１）各种细胞表面Ｂ７－１分子表达率分别 为： Ｕ１４， ５．２４％ ； Ｕ１４／ＬＮＳｍ Ｂ７， ５７．３９％ ； Ｕ１４／ｐＣＥＰｍ Ｂ７，４８．５２％ ；（２）各组小鼠肿瘤平均直径为：５×１０４组Ｕ１４，１．１９±０．４７ ｃｍ ；Ｕ１４／ＬＮＳｍ Ｂ７ ，０（Ｐ＜ ０．００１）；Ｕ１４／ｐＣＥＰｍ Ｂ７，０（Ｐ＜ ０．００１）。５×１０５ 组Ｕ１４，２．２６±０．９５ ｃｍ ?
Objective To study the immunogenicity of U14 cells in murine cervical cancer cells transfected with B7-1 gene. Methods (1) LNSm B7 and pCEPm B7 were transfected into U14 cells by virus infection and electroporation, respectively. Flow cytometry was used to detect the expression rate of B7-1 in positive mixed clonal cells U14/LNSm B7 and U14/pCEPm B7. (2) Different numbers of U14, U14/LNSm B7 and U14/pCEPm B7 cells were inoculated subcutaneously into Kunming mice, and after 4 weeks, the tumor size and pathological results of each group were observed; (3) 1×107 numbers were taken. After the mice were vaccinated with U14, U14/LNSm B7 and U14/pCEPm B7 twice, respectively, spleen T lymphocytes were isolated and cytotoxic T lymphocytes (CTLs) were tested for their in vitro cytotoxicity against U14 cells. . Results (1) The expression rates of B7-1 on various cell surfaces were: U14, 5.24%; U14/LNSm B7, 57.39%; U14/pCEPm B7, 48.52%; (2) Small groups The mean tumor diameters of the mice were: 5×104 groups U14, 1.19±0.47 cm; U14/LNSm B7, 0 (P<0.001); U14/pCEPm B7,0 (P<0.001). 5×105 group U14, 2.26±0.95 cm ?