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目的:探究采用高效液相色谱法同时测定三七伤药胶囊中4种成分(人参皂苷Rgl、柚皮苷、人参皂苷Rbl、芍药苷)的方法及效果。方法:采用shim-packVP-ODS C18(250mm×4.6mm,5μm)色谱柱;检测波长为230nm(0.01~20min)、283nm(20~35min)、203nm(35~100min);柱温为31℃;流速为1mL/min;乙腈-水(17∶83)作为流动相,梯度洗脱为17%乙腈,0~36min,17%~43%乙腈,37~81min,17%乙腈,82~100min。结果:三七伤药胶囊中4种成分在相应浓度内与峰面积保持良好的线性关系。结论:采用高效液相色谱法同时测定三七伤药胶囊中4种成分,方法简单、准确度高、灵敏度好、重复性良好,对控制三七伤药胶囊的质量可起到有效作用。
Objective: To explore the method and the effect of simultaneous determination of four components (ginsenoside Rgl, naringin, ginsenoside Rbl, paeoniflorin) in Sanqi Shangyao capsule by high performance liquid chromatography. METHODS: The chromatographic column was shim-pack VP-ODS C18 (250 mm × 4.6 mm, 5 μm). The detection wavelength was 230 nm (0.01-20 min), 283 nm (20-35 min) and 203 nm (35-100 min) The mobile phase was acetonitrile-water (17:83) with gradient elution of 17% acetonitrile, 0-36 min, 17-43% acetonitrile, 37-81 min, 17% acetonitrile, 82-100 min. RESULTS: The four components in Sanqi Shangyao Capsule maintained a good linear relationship with the peak area within the corresponding concentrations. Conclusions: Simultaneous determination of four components in Sanqi Shangyao Capsule by high performance liquid chromatography has the advantages of simple method, high accuracy, good sensitivity and good reproducibility, which can effectively control the quality of Sanchi Shangxue Capsule.