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目的:建立软坚消瘿颗粒中芍药苷、阿魏酸、橙皮苷的含量测定方法,为其质量标准的建立提供依据。方法:采用高效液相色谱波长切换法同时测定芍药苷、阿魏酸、橙皮苷含量,Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%冰醋酸(18∶82),流速1.0 mL·min-1,柱温25℃,检测波长0~12 min为230 nm,12~20 min为325 nm,20~40 min为283 nm。结果:3种活性成分实现良好分离,芍药苷在0.061 7~1.541 7 mg线性关系良好,r=0.999 9,平均回收率为97.25%,RSD 1.45%;阿魏酸在0.005 9~0.146 7 mg线性关系良好,r=0.999 7,平均回收率为98.66%,RSD 1.45%;橙皮苷在0.136 7~3.416 7 mg线性关系良好,r=0.999 6,平均回收率为97.55%,RSD 1.92%。结论:该方法简便、准确、可靠,重复性好,为控制软坚消瘿颗粒的质量提供依据。
OBJECTIVE: To establish a method for the determination of paeoniflorin, ferulic acid and hesperidin in Ruanjian Qiaoji granule, and provide the basis for the establishment of its quality standard. Methods: The contents of paeoniflorin, ferulic acid and hesperidin were determined simultaneously by high performance liquid chromatography with wavelength-switching method. The mobile phase consisted of acetonitrile-0.2% glacial acetic acid (18.5 mg × 250 mm, : 82), the flow rate was 1.0 mL · min-1 and the column temperature was 25 ℃. The detection wavelength was 230 nm at 0-12 min, 325 nm at 12-20 min and 283 nm at 20-40 min. Results: The three active constituents were well separated. The linear relationship of paeoniflorin was 0.061 7 ~ 1.541 7 mg, r = 0.999 9, the average recovery was 97.25%, RSD 1.45%. Ferulic acid was linear in 0.005 9 ~ 0.146 7 mg The average recovery was 98.66% and the RSD was 1.45%. The linearity of hesperidin was 0.136 7 ~ 3.416 7 mg, r = 0.999 6, the average recovery was 97.55% and the RSD was 1.92%. Conclusion: The method is simple, accurate, reliable and reproducible. It provides the basis for controlling the quality of granules in Ruanjian Qiaoji.