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作者报导了一种简单、敏感、可靠不用放射作用的新方法,来检验浓缩尿中的HCG,以诊断早孕。取20ml尿,通过一种特制的超微孔膜(浸渍分子分离器),在低压下用磁力搅拌器超速过滤(此膜可完全储留15000分子量之复合物,包括38000分子量的HCG),经2～3小时,将尿浓缩到2ml,浓缩尿再与β-hCG抗血清作普遍凝胶抑制妊娠试验。受试者为可凝早孕妇女共100例(临床最后证实均系妊娠),在闭经的不同期(1,2,3周)取尿标本,采用普通凝胶抑制妊娠试验,其中玻片法二组(Pregnosticon,Gravindex),试管法(HAI)一组。另取闭经1同2周血和尿标本以特殊的RIA(放射免疫法)作β-hCG分析,将上述方法(五组)与新法作比较。受检者不服任何药物。
The authors report a new, simple, sensitive and reliable method that does not require radiation to test for HCG in urine to diagnose early pregnancy. Take 20ml of urine, through a special microporous membrane (impregnated molecular separator), ultra-speed filtration with a magnetic stirrer under low pressure (this membrane can completely store 15000 molecular weight of the composite, including 38000 molecular weight HCG) 2 to 3 hours, the urine concentrated to 2ml, concentrated urine and then with β-hCG antiserum for general gel inhibition of pregnancy test. The subjects were women with congenital pregnancy, a total of 100 cases (clinically confirmed by the end of pregnancy are), in different stages of amenorrhea (1,2,3 weeks) to take urine samples, the use of ordinary gel inhibition of pregnancy test, Group (Pregnosticon, Gravindex), test tube method (HAI) a group. Another amenorrhea 1 with 2 weeks of blood and urine samples with a special RIA (radioimmunoassay) for β-hCG analysis, the above method (five groups) and the new method for comparison. The subjects refused to accept any drugs.