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目的探究2型糖尿病性勃起功能障碍(T2DED)大鼠模型的构建方法。方法 SD大鼠30只,随机分为两组:对照组10只,饲养普通饲料;实验组20只,饲养高脂饲料。饲养8周后,实验组腹腔注射小剂量链脲佐菌素(streptozotocin,STZ,30mg/kg),对照组注射同等剂量的柠檬酸钠-柠檬酸缓冲液。腹腔注射3d后采用剪尾法测定随机血糖,≥16.7mmol/L则认为2型糖尿病(T2DM)建模成功。糖尿病(DM)成模后于2、4、6、8周各测定一次随机血糖及体质量;于糖尿病成模后4、8周进行进行腹腔葡萄糖耐量试验(intraperitoneal glucose tolerance test,IPGTT),及腹腔胰岛素耐量试验(intraperitoneal insulin tolerance test,IPITT);于糖尿病成模后8周行阿扑吗啡(apomorphine,APO)实验,并测定每只大鼠的阴茎海绵体内压力(intracavemous pressure,ICP)。测压完成后,收集大鼠的血清,进行甘油三酯(triglyceride,TG),总胆固醇(total cholesterol,TC),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterin,LDL-C)含量的测量。结果(1)高脂喂养及小剂量STZ注射后,实验组20只大鼠有17只血糖>16.7mmol/L,与对照组相比,在注射后第2、4、6、8周实验组大鼠血糖均明显升高(P<0.05)。(2)IPGTT试验提示糖尿病大鼠存在胰岛素抵抗;IPITT试验提示糖尿病大鼠对胰岛素的敏感性下降。与对照组相比,实验组血清中的TG、TC、HDL-C、LDL-C含量显著升高(P<0.05);(3)APO实验提示对照组大鼠勃起率为100%,实验组中STZ注射后2只大鼠死亡,余15只大鼠有2只可观察到勃起。行ICP测定,实验组大鼠的ICP最大值明显低于对照组大鼠(P<0.05)。结论高脂喂养联合小剂量STZ注射可成功构建T2DED的大鼠模型,APO实验可以有效可靠地筛选ED动物模型。
Objective To explore a method for constructing a rat model of type 2 diabetic erectile dysfunction (T2DED). Methods Thirty SD rats were randomly divided into two groups: control group of 10, fed ordinary feed; experimental group of 20, fed high-fat diet. After 8 weeks of feeding, the experimental group was intraperitoneally injected with streptozotocin (STZ, 30mg / kg), while the control group was injected with the same dosage of sodium citrate-citrate buffer. After 3 days of intraperitoneal injection, random blood glucose was measured by tail-cut method, and type 2 diabetes (T2DM) was considered as successful if it was ≥16.7mmol / L. Diabetes mellitus (DM) were randomly divided into two groups: random blood glucose and body weight were measured at 2, 4, 6 and 8 weeks after diabetes mellitus; intraperitoneal glucose tolerance test (IPGTT) Intraperitoneal insulin tolerance test (IPITT) was performed. Apomorphine (APO) test was performed 8 weeks after diabetes mellitus, and the intracavemous pressure (ICP) of each rat was measured. After the pressure measurement was completed, the serum of the rats was collected and the triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein Cholesterol (low density lipoprotein cholesterin, LDL-C) measurement. Results (1) After high-fat diet and low-dose STZ injection, there were 17 blood glucose> 16.7mmol / L in 20 rats in the experimental group. Compared with the control group, Blood glucose in rats were significantly increased (P <0.05). (2) IPGTT test suggested insulin resistance in diabetic rats; IPITT test showed that insulin sensitivity of diabetic rats decreased. Compared with the control group, the levels of TG, TC, HDL-C and LDL-C in the serum of the experimental group were significantly increased (P <0.05); (3) The APO test showed that the erectile rate of the control group was 100% Two rats died after STZ injection, and two of the remaining 15 rats showed erection. By ICP, the maximal ICP value in the experimental group was significantly lower than that in the control group (P <0.05). Conclusion High-fat diet combined with low-dose STZ injection can successfully construct rat model of T2DED. APO can effectively and reliably screen ED animal models.