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目的:建立以高效液相色谱法测定贯叶连翘中金丝桃素含量的方法,并对不同产地贯叶连翘不同部位中金丝桃素的含量进行比较。方法:色谱柱为Boston pHlex ODSC18(150mm×4.6mm,5μm),流动相为甲醇-水(85:15,氨水调pH9.5),检测波长为588nm,流速为1.0mL·min-1。结果:金丝桃素检测浓度在0.14～3.60μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9997);平均回收率为100.7%,RSD=1.98%(n=6)。不同产地贯叶连翘不同部位中金丝桃素的含量在0.005～0.562mg·g-1之间。结论:不同产地贯叶连翘药材不同部位中金丝桃素含量均不同。本方法简便、准确、重现性好,可用于贯叶连翘的质量控制。
Objective: To establish a method for the determination of hypericin in Hypericum perforatum by high performance liquid chromatography (HPLC), and to compare the content of hypericin in different parts of Hypericum perforatum from different areas. Methods: The column was Boston pHlex ODSC18 (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-water (85:15, ammonia solution pH9.5). The detection wavelength was 588 nm and the flow rate was 1.0 mL · min-1. Results: The calibration curve of hypericin showed a good linear relationship with the peak area (r = 0.9997) in the range of 0.14 ~ 3.60μg · mL-1. The average recovery was 100.7% and RSD was 1.98% (n = 6) . Hypericin from different parts of Hypericum perforatum in different parts of the content of 0.005 ~ 0.562mg · g-1. Conclusion: The content of hypericin in different parts of Hypericum perforatum from different areas are different. The method is simple, accurate, reproducible, and can be used for quality control of Hypericum perforatum.