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本实验拟在体外培养大鼠肺微血管内皮细胞 (ＲＰＭＶＥＣｓ)的基础上 ,用免疫细胞化学Ｓ Ｐ法和免疫荧光细胞化学间接法检测ＲＰＭＶＥＣｓ膜Ｇ蛋白亚型。1 材料和方法1.1 材料 ＤＭＥＭ培养干粉 (美国ＧＩＢＩＣＯ公司 ) ;荧光 (ＦＩＴＣ)结合的羊抗兔抗体、兔抗大鼠Ｉｇ
In this study, based on the cultured rat pulmonary microvascular endothelial cells (RPMVECs) cultured in vitro, the protein subtypes of RPMVECs were detected by immunocytochemistry and indirect immunofluorescence cytochemistry. 1 Materials and methods 1.1 Materials DMEM cultured dry powder (GIBICO company, USA); FITC-conjugated goat anti-rabbit antibody, rabbit anti-rat Ig