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目的以离子凝胶法制备黄芩苷-血根碱离子对壳聚糖纳米粒(BSI-CS-NPs)。方法以单因素为主要考察方法,筛选最佳处方和制备工艺;采用透射电子显微镜(TEM)观察BSI-CS-NPs的形态,激光粒度分析仪测定粒径大小和Zeta电位,HPLC法检测包封率和载药量。结果所制BSI-CS-NPs外观圆整,粒度分布均匀,平均粒径为326.4 nm,Zeta电位为45.7 mV,包封率为68.73%,载药量为26.68%。相比黄芩苷-血根碱离子对原料药,BSI-CS-NPs 2 h的药物累积释放率减少了约36.51%,12 h累积释放率为92.29%。结论离子凝胶法适用于BSI-CS-NPs的制备,且具有缓释性能。
Objective To prepare baicalin-hemin alkali-chitosan nanoparticles (BSI-CS-NPs) by ion-gel method. Methods Single factor was used as the main method to screen the best prescription and preparation process. The morphology of BSI-CS-NPs was observed by transmission electron microscopy (TEM), the particle size and Zeta potential were measured by laser particle size analyzer, Rate and drug loading. Results The appearance of BSI-CS-NPs was uniform and the particle size distribution was uniform. The average particle size was 326.4 nm, the zeta potential was 45.7 mV, the entrapment efficiency was 68.73%, and the drug loading was 26.68%. The cumulative release rate of BSI-CS-NPs by 2 h was 36.51% less than that of baicalin-sanguin ion, and the cumulative release rate of BSI-CS-NPs was 92.29% after 12 h. Conclusion Ion-gel method is suitable for the preparation of BSI-CS-NPs with sustained release properties.