论文部分内容阅读
目的 建立一种符合临床垂体腺瘤生物学特征的动物模型。方法 体外培养大鼠恶性垂体腺瘤细胞 (mGH3) ,通过立体定向将mGH3细胞 1× 1 0 9个 /L移植至大鼠 1侧侧脑室 ,病理检查移植瘤生长情况 ,放射免疫法监测循环中大鼠生长激素 (rGH)水平。结果 体外培养的mGH3细胞具有分泌rGH的能力 ,大鼠脑室内接种mGH3细胞后 3周 ,出现肢端肥大、尾巴增粗、毛发增多、结膜水肿等改变 ,荷瘤鼠平均生存 ( 4 4.2± 5.6)d ,肿瘤向周围脑组织浸润性生长 ,肿瘤细胞异形性明显 ,肿瘤新生血管丰富 ;从第 3周起大鼠循环中rGH水平逐渐升高直至死亡。结论 同种移植性大鼠恶性垂体腺瘤具有浸润性、高血运性和释放生长激素的能力 ,是一种较理想的垂体腺瘤动物模型
Objective To establish an animal model that meets the biological characteristics of clinical pituitary adenomas. Methods Rat malignant pituitary adenoma cells (mGH3) were cultured in vitro. mGH3 cells were transplanted to the lateral ventricle of rats by stereotactic treatment. The growth of the transplanted tumors was examined by pathology. Radioimmunoassay was used to monitor the circulation. Rat growth hormone (rGH) levels. Results mGH3 cells cultured in vitro had the ability to secrete rGH. Three weeks after inoculation of mGH3 cells in the rat’s cerebellum, acromegaly, thickening of the tail, hair growth, conjunctival edema, and other changes occurred, and the average survival of the tumor-bearing mice was 4 4.2± 5.6. )d , tumors infiltrative growth to the surrounding brain tissue, tumor cell heterogeneity, tumor neovascularization rich; from the third week of the rat circulating rGH levels gradually increased until death. Conclusion The same kind of transplanted rat malignant pituitary adenoma has invasive, high blood flow and the ability to release growth hormone. It is an ideal animal model of pituitary adenoma.