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在徐淑云介绍的用新鲜鸡红细胞(CRBC)作为吞噬物,半体内法测定小鼠巨噬细胞(Mφ)吞噬功能的基础上,我们进行了改进。其一将CRBC进行醛化。取阿氏液抗凝的新鲜CRBC用生理盐水离心洗2次,1ml压积的CRBC中加入24ml生理盐水、0.1ml 25％戊二醛,吹打均匀,室温下摇动30分钟,用生理盐水洗3次,4℃可存1年,使用时配成1％浓度;其二省去Mφ与吞噬物在玻片上于37℃温育使之贴壁的步骤,而将1％醛化CRBC注入小鼠腹腔,待作用1小时后,处死小鼠、用洁净玻片在暴露的腹
In Xu Shuyun introduction of fresh chicken erythrocytes (CRBC) as a phagocytosis, semi-in vivo method of measuring mouse macrophage (Mφ) phagocytosis based on the foundation, we have improved. One of the CRBCs is hydroformylated. Take fresh anticoagulant CRBC Centrifugal wash 2 times with saline, 1ml pressure CRBC added 24ml saline, 0.1ml 25% glutaraldehyde, whipped evenly, shake at room temperature for 30 minutes, washed with saline 3 Times, 4 ℃ can be stored for 1 year, when used with 1% concentration; the second omitted Mφ and phagocytosis on glass slides incubated at 37 ℃ to make it adhere to the step, and 1% of the aldehyde CRBC injected mice Abdominal cavity, to be treated after 1 hour, mice were sacrificed, with a clean glass slide in the exposed belly