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目的采用指纹图谱与一测多评(QAMS)结合的方法评价荔枝多酚提取物,并测定提取物中4种多酚类成分。方法采用RP-UPLC法测定22批荔枝多酚提取物,建立指纹图谱的共有模式。以表儿茶素为内参物,建立原花青素A2、原花青素B2、表儿茶素-(4β→8,2β→O→7)-表儿茶素-(4β→8)-表儿茶素(PC-C)的相对校正因子(f),并测定各成分量,实现QAMS。同时比较QAMS和外标法测定4种多酚类成分量测得值的差异,验证QAMS的可行性及其准确性。结果 22批荔枝多酚提取物特征指纹图谱标定了19个共有峰,指认了其中9个共有峰,即4个已知主成分6号峰(原花青素B2)、8号峰(表儿茶素)、9号峰(PC-C)、15号峰(原花青素A2),3个A型原花青素三聚体(12、16、17号峰),1个A型原花青素二聚体(19号峰)和1个B型原花青素二聚体(14号峰);22批荔枝多酚提取物相似度大于0.9,其中4个主成分的QAMS计算值与外标法实测值间无显著差异(P>0.25)。结论指纹图谱与QAMS结合的质控模式准确可行,可为全面合理评价荔枝多酚提取物的质量提供参考。
OBJECTIVE To evaluate the polyphenol extract of litchi by using a combination of fingerprinting and multiple test (QAMS), and to determine the contents of four polyphenols in the extract. Methods RP-UPLC method was used to determine the total of 22 lotus polyphenol extracts and establish a common pattern of fingerprints. The epicatechin A2, proanthocyanidin B2, epicatechin - (4β → 8,2β → O → 7) - epicatechin - (4β → 8) - epicatechin -C) relative correction factor (f), and determination of the amount of each component to achieve QAMS. At the same time, the differences of the measured values of four polyphenols from QAMS and external standard method were compared to verify the feasibility and accuracy of QAMS. Results Nineteen common peaks were identified from the fingerprint of polyphenol extracts from 22 batches of lychee. Nine common peaks were identified, namely, four known major components (proanthocyanidin B2), peak 8 (epicatechin) (PC-C), Peak 15 (proanthocyanidin A2), three A-type proanthocyanidin trimers (peaks 12,16 and 17) and one type A proanthocyanidin dimer (peak 19) 1 B-type proanthocyanidin dimer (peak 14). The similarity of 22 loti polyphenol extracts was greater than 0.9. There was no significant difference (P> 0.25) between the QAMS values of the four principal components and those of external standard method . Conclusion The quality control mode of QAMS fingerprinting is accurate and feasible, which can provide a reference for the comprehensive and reasonable evaluation of litchi polyphenol extract quality.