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目的通过对新疆哈萨克族健康人群和食管癌患者放化疗前后血清蛋白质组特征的研究,探讨放化疗对食管癌患者相关蛋白质表达谱的影响。方法收集、制备哈萨克族食管癌治疗前后患者组(10例)和健康对照组(10例)血清样品,采用蛋白质二维液相色谱系统(Proteome LabTMPF-2D)对治疗前后组和正常人群组三组血清样品进行分离与数据比较分析。结果哈萨克族食管癌患者放化疗前后相比存在21个显著差异的表达蛋白位点峰值;而患者治疗前和健康对照组对比有10个存在显著差异的表达蛋白位点峰值,患者治疗后和健康对照组有4个存在显著差异的表达蛋白位点峰值。结论血清中可以筛选到对新疆哈萨克族食管癌患者诊断及判断放化疗有意义的特异性蛋白表达位点。
Objective To investigate the effect of radiotherapy and chemotherapy on the protein profiles of patients with esophageal cancer by analyzing the characteristics of serum proteome in patients with Kazakh and Kazakh before and after radiotherapy and chemotherapy. Methods Serum samples of patients with esophageal cancer before and after treatment (10 cases) and healthy controls (10 cases) were collected and analyzed. Proteome LabTMPF-2D Three groups of serum samples were compared with the data analysis. Results There were 21 differentially expressed protein peaks in patients with esophageal cancer before and after radiotherapy and chemotherapy. There were 10 peaks in the expression of protein in patients with esophageal cancer before and after radiotherapy and chemotherapy. There were 4 significant differences in the expression of the protein spots in the control group. Conclusion Serum can be screened to Xinjiang Kazakh ethnic patients with esophageal cancer diagnosis and judgment radiotherapy and chemotherapy of specific protein expression sites.