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目的 了解 L6 15白血病细胞株能否在 (C5 7BL/ 6× 6 15 ) F1小鼠体内建立白血病模型。方法 雄性 6 15小鼠与雌性 C5 7BL / 6小鼠采用长期同居法培育 (C5 7BL / 6× 6 15 ) F1小鼠 ,6 15、F1小鼠分别经腹腔接种不同数量 L 6 15白血病细胞株 ,观察其白血病发病情况 ;取发病濒死 F1小鼠脾脏 ,制备脾细胞悬液 ,按不同数量经腹腔注射分别接种予 F1小鼠 ,观察白血病发病情况。结果 (C5 7BL/ 6× 6 15 ) F1小鼠接种 L6 15白血病细胞后 10 0 %发病 ,L6 15白血病细胞可在 (C5 7BL/ 6× 6 15 ) F1小鼠体内传代。结论 L6 15白血病细胞株能在 (C5 7BL/ 6× 6 15 ) F1小鼠体内成功接种并传代
Objective To investigate whether L6 15 leukemia cell line can establish leukemia model in (C5 7BL / 6 × 6 15) F1 mice. Methods Male mice and female C5 7BL / 6 mice were inoculated intraperitoneally with different numbers of L 6 15 leukemia cell lines (C5 7BL / 6 × 6 15), F1 mice , To observe the incidence of leukemia; taking the morula of dying F1 mouse spleen to prepare spleen cell suspension, and then inoculating F1 mice according to different quantity through intraperitoneal injection to observe the incidence of leukemia. Results (C5 7BL / 6 × 6 15) F1 mice were challenged with 10% L6 15 leukemia cells. L6 15 leukemia cells were passaged in (C5 7BL / 6 × 6 15) F1 mice. Conclusion L6 15 leukemia cell line can be successfully inoculated and passaged in (C5 7BL / 6 × 6 15) F1 mice