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由尖孢镰刀菌古巴专化型(Fusarium oxysporum fsp.cubense)引起的香蕉枯萎病严重阻碍着香蕉产业的可持续发展。为探索建立一种快捷的测定致病力的方法,以来自广东、海南的9株分离株的致病力测定为基础,8株香蕉枯萎病4号小种为研究对象,1株1号小种为对照,比较了水培苗直接浇菌法、盆栽苗伤根淋菌法、椰糠杯苗伤根浇菌法3种方法。研究发现各菌株在3种测定方法中表现的致病力强弱程度基本一致,其中以盆栽苗伤根淋菌法处理的9株病原菌间的致病力差异最大:水培苗直接浇菌法比其他2种方法操作简便且节省空间,致病周期与其他2种方法相当或更短。筛选出的水培苗直接浇菌法应更适用于分析大批量菌株间的致病力差异性,该方法操作简便,有助于病原菌致病相关基因功能分析和抗病香蕉品种选育等研究。
Banana wilt disease caused by Fusarium oxysporum fsp.cubense seriously hinders the sustainable development of banana industry. In order to explore a rapid method for the determination of pathogenicity, based on the pathogenicity determination of 9 isolates from Guangdong and Hainan, 8 banana wilt disease races were studied, and 1 strain No.1 was small As the control, we compared the methods of directly fertilizing the hydroponic plantlets, the root gonococcus transplanting method of potted seedlings, The results showed that the pathogenicity of each strain in the three methods was basically the same, among which the pathogenicity difference among the nine pathogenic bacteria treated by root nodulation of pot plant seedlings was the largest: The other two methods are easy to operate and space-saving, and the disease cycle is comparable to or shorter than the other two methods. The direct selection of hydroponics seedling sterilization method should be more suitable for the analysis of high virulence strains of pathogenicity differences, the method is simple and convenient, is conducive to pathogenesis of disease-related genes and disease-resistant banana variety breeding studies .