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本研究采用原位杂交技术,探讨精制血府胶囊对与心肌缺血密切相关的基因表达的影响,阐明其抗心肌缺血的分子机制。结果:精制血府胶囊、血府逐瘀胶囊,硫氮革酮等三种“药血清”组的LDH、CK、COT释放量与同浓度的兔血清组比较均有极显著差异(p<0.01)。精制血府胶囊组、硫氮草酮组抑制LDH的释放较血府逐瘀胶囊组显著(p<0.01)。原位杂交显示:ET-1的基因杂交结果,精制血府胶囊组与正常培养细胞组均未见杂交颗粒,硫氮草酮组与缺氧缺糖组心肌细胞核周围胞质中可见大量杂交颗粒;血府逐瘀胶囊组,兔血清组仅见少量杂交颗粒。ECE-1的基因杂交结果,缺氧缺糖组,兔血清组与硫氮草酮组心肌细胞胞质中均可见大量杂交颗粒,且三组无明显差异;精制血府胶囊组、血府逐瘀胶囊组、正常培养组均未见阳性杂交信号。结果表明,精制血府胶囊具有心肌细胞保护作用,并与其抑制ET-1,ECE-1的基因表达有关。
In this study, in situ hybridization was used to investigate the effect of purified Xuefu Capsule on gene expression closely related to myocardial ischemia and to elucidate its molecular mechanism against myocardial ischemia. Results: The levels of LDH, CK, and COT in the three drug serum groups such as refined Xuefu capsules, Xuefu Zhuyu capsules and thiazolone were significantly different from those of rabbit serum samples with the same concentration (p<0.01). ). The release of LDH from the refined Xuefu capsule group and sulfatrione group was significantly higher than that of Xuefu Zhuyu capsule group (p<0.01). In situ hybridization showed that ET-1 gene hybridization results showed that there were no hybrid particles between the purified Xuefu capsule group and the normal cultured cell group, and there were a large number of hybrid particles in the nucleus cytoplasm of the thiuracatone group and the hypoxia-glucose deprived group. In the Xuefu Zhuyu capsule group, only a small amount of hybrid particles was found in the rabbit serum group. ECE-1 gene hybridization results, hypoxia-hypoglycemia group, rabbit serum group and thiuratone group can see a large number of hybrid particles in the cytoplasm, and there was no significant difference between the three groups; refined Xuefu capsule group, Xuefu by No positive hybridization signal was observed in the capsule group and the normal culture group. The results showed that the purified Xuefu capsule had cardioprotective effects and was related to its inhibition of ET-1, ECE-1 gene expression.