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目的探讨体外培养的自体内皮祖细胞(EPC)对兔颈动脉球囊损伤后内皮修复以及内膜增生的影响。方法分离兔骨髓单个核细胞,EBM-2+SingleQuots 培养基培养,鉴定 EPC 表面标志,并标记。建立兔颈动脉球囊损伤模型,在动脉球囊损伤的即刻,将体外培养的自体 EPC 经损伤动脉缓慢注入动物体内。分别在手术后7、14天处死动物。检测损伤部位的内皮修复以及内膜增生情况,计算内膜中膜比值(I/M 比值)并检测注入的细胞在血管损伤局部的分布。结果 EPC 治疗组在7、14天的内皮覆盖率分别为(50.923±2.476)%和(82.609±2.611)%,均明显高于对照组,差异有统计学意义。EPC 治疗组14天的 I/M 比值为0.378±0.029,低于对照组,差异有统计学意义。免疫组织化学染色示 EPC 治疗组损伤血管局部有标记的 EPC 分布。结论内皮球囊损伤后动脉内注入EPC 能有效促进内皮修复,减少内膜增生,预防再狭窄的发生。
Objective To investigate the effect of endothelial progenitor cells (EPC) cultured in vitro on endothelial repair and intimal hyperplasia after carotid artery balloon injury in rabbits. Methods Rabbit bone marrow mononuclear cells were isolated and cultured on EBM-2 + SingleQuots medium. EPC surface markers were identified and labeled. Rabbit carotid artery balloon injury model was established. At the moment of arterial balloon injury, autologous EPC cultured in vitro was slowly injected into animals through the damaged artery. Animals were sacrificed 7, 14 days after surgery. Endothelial repair and intimal hyperplasia were detected at the injury site. The intima-media ratio (I / M ratio) was calculated and the distribution of the implanted cells in the local area of vascular injury was examined. Results The endothelial cell coverage of EPC-treated group was (50.923 ± 2.476)% and (82.609 ± 2.611)% at 7 and 14 days, respectively, which were significantly higher than that of the control group. The difference was statistically significant. The 14-day I / M ratio of EPC-treated group was 0.378 ± 0.029, which was lower than that of the control group, the difference was statistically significant. Immunohistochemical staining showed that the EPC-treated EPCs had marked EPC distribution in injured blood vessels. Conclusion Intra-arterial injection of EPC after endothelial balloon injury can effectively promote endothelial repair, reduce intimal hyperplasia and prevent restenosis.