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慢性活动性肝炎(CAH)和原发性胆汁性肝硬化(PBC)病人血清中常含高滴度自身抗体,其中某些抗体如平滑肌抗体可与细胞支架包括微丝、中间丝和微管起反应。本文作者用长春花硷处理、培养的人胚胎成纤维细胞作为基质,建立了一种能分别检测抗微丝、中间丝和微管的抗体的间接免疫荧光技术,对24例CAH(内1例HBsAg阳性,余均阴性)、15例PBC和20例酒精性肝病(ALD)患者,以及32例健康人的血清中这三种细胞支架抗体出现情况作了研究。
Serum of patients with chronic active hepatitis (CAH) and primary biliary cirrhosis (PBC) often contains high titers of autoantibodies, some of which, such as smooth muscle antibodies, react with cell scaffolds including microfilaments, intermediate filaments and microtubules . In this study, we used an indirect immunofluorescence technique to detect anti-microfilament, intermediate filament, and microtubule-specific antibodies using vinblastine-treated and cultured human embryonic fibroblasts as a substrate. 24 cases of CAH (1 case HBsAg positive, the remaining negative), 15 cases of PBC and 20 cases of alcoholic liver disease (ALD) patients, as well as the serum of 32 healthy subjects in these three kinds of cell scaffold antibodies were studied.