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目的 深入了解HIV在灭活因素作用下包膜基因变异。方法 对HIV - 1B3亚型毒株在S/D法及低pH法灭活作用前后的样品 ,套式PCR扩增其包膜基因C2～C3区 5 6 4bp的核酸片段 ,进行核苷酸序列测定和分析。结果 两种方法灭活前后 ,包膜基因变异均不显著 ,核苷酸同源性均为 98%。结论 S/D法及低 pH法灭活作用前后HIV包膜基因变异不显著。体外传代HIV包膜基因趋于稳定
Objective To understand the genetic variation of envelope gene under the action of inactivation factor in HIV. Methods The samples of HIV - 1B3 subtype strain before and after inactivation by S / D method and low pH method were enrolled in this study. A 56 bp DNA fragment of C2 ~ C3 of the envelope gene was amplified by nested PCR. The nucleotide sequence Determination and analysis. Results Before and after inactivation of the two methods, there was no significant variation in the envelope gene, nucleotide homology was 98%. Conclusion There was no significant difference in HIV envelope gene before and after S / D and low pH inactivation. In vitro passage of HIV envelope gene tends to be stable