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应用三种炭疽快速检验法(荚膜荧抗法、串珠湿片法及串珠荧抗法),检查了炭疽疫区人畜病理标本141份,非炭疽对照标本82份,常规细菌培养作为对照方法。三种快速法检查对照标本均未发现阳性,说明它们的特异性均较好。检验阳性率:荚膜荧抗法为59.6％,串珠湿片法为45.4％,串珠荧抗法为45.3％,常规培养法为61％。快速法中荚膜荧抗法阳性率最高,检验只需2小时,串珠湿片法次之,检验时间为5小时,操作简易,无需特殊设备。文中还介绍了二种标本保存法:①中性福尔马林固定薄片法,有利荧光抗体检查;②琼脂或淀粉管保存法,使细菌转变成芽胞,以利作串珠湿片法和培养。
Three methods of anthrax rapid test (capsular fluorescence method, beads wet method and bead-fluorescence method) were used to examine 141 pathological specimens of human and livestock in anthrax epidemic area and 82 non-anthrax control specimens. Conventional bacterial culture was used as the control method. Three fast test methods were not positive control specimens, indicating that their specificity are better. The test positive rate: capsular fluorescence method was 59.6%, beads wet sheet method was 45.4%, beaded fluorescence method was 45.3%, conventional culture method was 61%. Fast method in the highest positive rate of capsular fluorescence method, the test only 2 hours, beaded wet method second, the test time is 5 hours, easy to operate, no special equipment. The article also introduced two kinds of specimen preservation method: ① neutral formalin fixed sheet method, favorable fluorescent antibody examination; ② agar or starch tube preservation method, so that bacteria into spores, to facilitate the beads wet method and culture.