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目的 观察小鼠实验中免疫佐剂QS 2 1对乙型肝炎核心抗原 (HBcAg)核酸疫苗免疫应答的增强作用。方法 基因枪轰击法接种HBcAg核酸疫苗或对照质粒 ,皮内注射法给予QS 2 1;间接酶联免疫吸附试验 (ELISA)法检测小鼠血清抗 HBc(IgG及IgG亚类 )水平 ;51铬释放法检测小鼠脾细胞特异性CTL杀伤活性。结果 Balb/c和C5 7BL/ 6小鼠单用疫苗组和疫苗加QS 2 1组的血清抗 HBc终点滴度均分别为 1∶32 80 5 0和 1∶98415 0 ;两组的抗 HBc IgG亚类均以IgG2a略占优势 ;当效∶靶比为 12∶1时 ,Balb/c小鼠单用疫苗组和疫苗加QS 2 1组的特异性CTL杀伤活性分别为 5 1.1%和6 3 .6 % ;C5 7BL/ 6小鼠两组的CTL杀伤活性分别为 5 3 .1%和 6 8.1%。结论 QS 2 1对HBcAg核酸疫苗所诱导的小鼠特异性抗体及CTL应答均有增强作用
Objective To observe the enhancing effect of immune adjuvant QS 2 1 on the immune response of hepatitis B core antigen (HBcAg) DNA vaccine in mice. Methods HBsAg DNA vaccines or control plasmids were inoculated by gene gun bombardment and administered with QS 2 1 intradermally. The level of serum anti-HBc (IgG and IgG subclasses) in mice was detected by indirect enzyme-linked immunosorbent assay (ELISA) Method to Detect Specific CTL Killing Activity of Mouse Spleen Cells. Results The final anti-HBc titers of both Balb / c and C5 7BL / 6 mice in vaccine group and vaccine plus QS 2 1 group were 1:32 80 5 0 and 1:98415 0 respectively. The anti-HBc IgG The specific CTL killing activity of Balb / c mice with vaccine group and vaccine plus QS 2 1 group was 51.1% and 63%, respectively .6%. The CTL killing activity of C5 7BL / 6 mice was 53.1% and 6 8.1% respectively. Conclusion QS 2 1 enhances the specific antibody and CTL response induced by HBcAg DNA vaccine in mice