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NUCC-3 choriocarcinoma cell line was used as an in vitro placental cell model to investigate the effects of follistatin on GnRH-stimulated hCG secretion and its subunit mRNA level and stability. Follistatin alone did not affect basal hCG secretion and its subunit mRNA level. GnRH increased hCG secretion and hCG β-mRNA level in a dose-dependent manner. Follistatin significantly suppressed GnRH-stimulated hCG secretion and hCG α- and β-mRNA level. It inhibited hCG secretion in response to PMA, and did not affect the stability of hCG α- and β-mRNA. The authors suggest that follistatin inhibits GnRH-stimulated hCG secretion as well as hCG α- and β-RNA level by decreasing the rate of transcription through the second messenger transduction system--protein kinase-C.
NUCC-3 choriocarcinoma cell line was used as an in vitro placental cell model to investigate the effects of follistatin on GnRH-stimulated hCG secretion and its subunit mRNA level and stability. Follistatin alone did not affect basal hCG secretion and its subunit mRNA level. GnRH Increased hCG secretion and hCG β-mRNA levels in a dose-dependent manner. Follistatin significantly suppressed GnRH-stimulated hCG secretion and hCG α- and β-mRNA levels. It inhibited hCG secretion in response to PMA, and did not affect the stability of hCG α and β-mRNA. The authors suggests that follistatin inhibits GnRH-stimulated hCG secretion as well as hCG α and β-RNA levels by decreasing the rate of transcription through the second messenger transduction system - protein kinase-C.