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将灭活的SARS冠状病毒抗原每隔两周多点注射免疫兔,共免疫3次,以观察SARS灭活病毒免疫兔后兔血清中IgG特异性抗体的应答变化。免疫前及第一次免疫后第8、14、21、28、35天耳静脉取血,分离血清。间接ELISA法测得血清中特异性IgG抗体持续升高,并表现出一定的剂量依赖性。第35天G1、G2、G3组血清IgG抗体滴度分别为1∶51200、1∶49600、1∶25600;中和试验测得G1组第28天血清样品中和抗体效价为1∶2560;蛋白芯片测得M、N、3CL、S1、S2、S3、S4等病毒蛋白抗原都可特异性结合抗血清中的IgG抗体,但是不同蛋白抗原结合能力有差别。因此可认为SARS灭活病毒经皮下注射免疫兔后,可诱导全身性IgG抗体应答,产生SARS冠状病毒特异性抗体。
The inactivated SARS coronavirus antigens were injected into the rabbits at every two weeks for three times to observe the changes of the IgG-specific antibodies in rabbit serum after the SARS-inactivated rabbits were immunized. Blood was collected from the ear vein on the 8th, 14th, 21st, 28th, and 35th day after immunization and after the first immunization, and the serum was separated. Indirect ELISA measured serum IgG antibodies continued to rise, and showed a dose-dependent manner. On day 35, the serum IgG antibody titers in G1, G2 and G3 groups were 1: 51200, 1: 49600 and 1: 25600, respectively. The neutralization test showed that the serum antibody titer in serum of G1 group was 1:2560 on the 28th day. Protein chip measured M, N, 3CL, S1, S2, S3, S4 and other viral protein antigens can specifically bind to the anti-serum IgG antibodies, but different proteins have different antigen binding capacity. Therefore, SARS coronavirus specific antibodies can be generated after immunization of rabbits with subcutaneous injections of SARS inactivated virus to induce systemic IgG antibody responses.