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目的:本研究旨在探讨:①健脾化瘀方体外对人肝癌细胞株HepG2增殖、黏附和侵袭的生物行为学影响;②健脾化瘀方体外对人肝癌细胞株HepG2凋亡的影响。方法:①采用MTT法、黏附实验、Transwell小室侵袭迁移实验,观察不同浓度的健脾化瘀方对人肝癌细胞株HepG2增殖、黏附和侵袭的影响;②利用流式细胞仪,AnnexinV/PI双染色法,研究不同浓度健脾化瘀方对人肝癌细胞株HepG2早期凋亡的诱导作用。结果:①健脾化瘀方有抑制人肝癌细胞株HepG2增殖的作用,并呈一定的浓度依赖性:同一时间不同浓度各组细胞的生长抑制率有明显差异(P<0.01)。②健脾化瘀方高、中浓度(2、1mg/mL)作用HepG 2细胞40、60和120min时,细胞的黏附能力明显降低(P<0.01)。健脾化瘀方作用HepG 2细胞24h后,侵袭的细胞数减少(P<0.05)。③健脾化瘀方对人肝癌细胞HepG2作用48h后,有诱导人肝癌细胞株HepG2凋亡的作用(P<0.05)。结论:①健脾化瘀方对人肝癌细胞株HepG2的增殖具有抑制作用;②健脾化瘀方能抑制人肝癌细胞株HepG2的黏附能力和侵袭能力;③健脾化瘀方有诱导人肝癌细胞株HepG2凋亡的作用。
Objective: The purpose of this study is to investigate: ① the biological behavior of Jianpi Huayu Fang on proliferation, adhesion and invasion of human hepatocellular carcinoma cell line HepG2 in vitro; ② the effect of Jianpi Huayu Fang on apoptosis of human hepatocellular carcinoma cell line HepG2 in vitro. Methods: ① The effects of different concentrations of Jianpi Huayu Recipe on the proliferation, adhesion and invasion of human hepatocellular carcinoma cell line HepG2 were observed by MTT assay, adhesion assay and Transwell cell invasion and migration assay; ②Tumor necrosis factor- Staining method to study the different concentrations of Jianpi Huayu Fang on human hepatoma cell line HepG2 early apoptosis induction. Results: ① Jianpi Huayu Recipe can inhibit the proliferation of HepG2 cells in a concentration-dependent manner. The growth inhibition rates of cells in different concentrations at the same time were significantly different (P <0.01). ② The adhesion capacity of HepG 2 cells decreased significantly at 40, 60 and 120 min (2, 1 mg / mL) of Jianpi Huayu formula (P <0.01). The effect of Jianpi Huayu Fang on HepG2 cells decreased after 24h (P <0.05). ③ The effect of Jianpi Huayu Recipe on HepG2 cells induced apoptosis of human hepatocellular carcinoma cell line HepG2 (P <0.05). Conclusion: ① Jianpi Huayu Recipe can inhibit the proliferation of HepG2 cells; ② Jianpi Huayu Recipe can inhibit the adhesion and invasion ability of HepG2 cells; ③ Jianpi Huayu Recipe can induce human hepatocellular carcinoma The role of apoptosis in HepG2 cells.