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AIM:To explore the abnormal function of platelets and therole of angelica sinensis injection(ASI)in patients withulcerative colitis(UC).METHODS:In 39 patients with active UC,25 patients withremissive UC and 30 healthy people,α-granule membraneprotein(GMP-140)and thromboxane B_2(TXB_2)weredetected by means of ELISA,6-keto-PGF_(1a)was detected byradioimmunoassay,platelet count(PC)and 1 min plateletaggregation rate(1 min PAR)were detected by bloodautomatic tester and platelet aggregation tester respectively,and yon Willebrand factor related antigen(vWF:Ag)wasdetected by the means of monoclonal-ELISA.The 64 patientswith UC were divided into two therapy groups.After routinetreatment and angelica sinensis injection(ASI)+routinetreatment respectively for 3 weeks,all these parameterswere also detected.RESULTS:The PC,1 min PAR and levels of GMP-140,TXB_2,and vWF:Ag in active UC were significanrly higherthan those in remissive UC and normal controls(P<0.05-0.01).Meanwhile,1 min PAR and levels of GMP-140,TXB_2,and vWF:Ag in remissive UC were still significantly higherthan those in normal controls(P<0.05).Furthermore,6-keto-PGF_(1a)level in active and remissive UC was remarkablylower than that in normal control(P<0.05-0.01).Theseparameters except 6-keto-PGF_(1a)were significantly improvedafter the treatment in ASI therapy group(P<0.05-0.01),whereas they all were little changed in routine therapygroup(P>0.05).CONCLUSION:Platelets can be significantly activated inUC,which might be related with vascular endothelium injuryand imbalance between TXB_2 and 6-keto-PGF_(1a)in blood.ASI can significantly inhibit platelet activation,relievevascular endothelial cell injury,and improve microcirculationin UC.
AIM: To explore the abnormal function of platelets and therole of angelica sinensis injection (ASI) in patients with ulcerative colitis (UC). METHODS: In 39 patients with active UC, 25 patients withremissive UC and 30 healthy people, alpha-granule membrane protein -140) and thromboxane B_2 (TXB_2) weredetected by means of ELISA, 6-keto-PGF_ (1a) was detected by radioactive immunoassay, platelet count (PC) and 1 min plateletaggregation rate ELISA was performed on a monoclonal-ELISA. The 64 patients with UC were divided into two therapy groups. After routine treatment and angelica sinensis injection (ASI) + routinetreatment respectively for 3 weeks, all these parameterswere also detected.RESULTS: The PC, 1 min PAR and levels of GMP-140, TXB_2, and vWF: Ag in active UC were significantly higher than those in remissive UC and normal controls (P <0.05-0.01) 1 min PAR and levels o fürthermore, 6-keto-PGF_ (1a) level in active and remissive UC was remarkablylower than that in normal (fPMP-140, TXB_2, and vWF: Ag in remissive UC were still significantly higherthan those in normal controls (P <0.05-0.01). The separameters except 6-keto-PGF_ (1a) were significantly improved after the treatment in ASI therapy group (P <0.05-0.01) . CONCLUSION: Platelets can be significantly activated in UC, which might be related with vascular endothelium injury and imbalance between TXB_2 and 6-keto-PGF_ (1a) in blood. ASI can significantly inhibit platelet activation, relieve vascular endothelial cell injury, and improve microcirculation in UC.