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目的动态观测UC大鼠结肠黏膜IL-1βmRNA表达的变化及药物的影响。方法采用TNBS灌肠法复制UC大鼠模型,并采用逆转录-聚合酶链反应(RT-PCR)的方法半定量检测结肠黏膜IL-1βmRNA的表达。结果与正常组比较,模型大鼠结肠黏膜IL-1βmRNA表达在3,7,14 d续增高(P<0.01);与模型组比较,溃结灵或SASP在3,14 d可显著降低结肠黏膜致炎因子IL-1βmRNA的表达(P<0.05,P<0.01),而在7 d时作用不明显。结论致炎因子IL-1β在TNBS急性UC模型中持续参与炎症及结肠黏膜的损伤过程;在炎症发生发展的过程中,早期是药物干预的关键时期,而恢复期同样需要持续用药。
Objective To observe the changes of IL-1β mRNA expression in colonic mucosa of UC rats and the effect of drugs. Methods The UC rats model was reproduced by TNBS enema, and the expression of IL-1βmRNA in colonic mucosa was detected semiquantitatively by reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with the normal group, the expression of IL-1βmRNA in the colonic mucosa of the model rats continued to increase at 3,7,14 d (P<0.01). Compared with the model group, Kuijieling or SASP significantly decreased the colon mucosa at 3, 14 d. The expression of IL-1β mRNA was significantly increased (P<0.05, P<0.01), but it was not significant at 7 days. Conclusion IL-1β, a pro-inflammation factor, is involved in inflammation and the damage of colonic mucosa in TNBS acute UC model. In the process of inflammation, early stage is the key period for drug intervention, and the recovery period also requires continuous medication.