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目的：研究不同造血生长因子联合应用对骨髓粒－单系造血祖细胞体外增殖的调节作用。方法：应用甲基纤维素体外半固体培养法，检测各种生长因子联合应用组，ＣＦＵ－ＧＭ集落的生长情况。结果：造血生长因子的联合应用不仅可增加ＣＦＵ－ＧＭ集落数，促进ＣＦＵ－ＧＭ集落的体外形成，而且，也能明显地增加ＣＦＵ－ＧＭ集落的体积，大型ＣＦＵ－ＧＭ集落数的比例在各联合应用组增加显著，其中以ＩＬ－３＋ＩＬ－６＋Ｇ－ＣＳＦ＋ＧＭ－ＣＳＦ联合应用组作用最强。同时，结果还显示，造血生长因子的联合应用可加速ＣＦＵ－ＧＭ集落的形成。结论：造血生长因子的联合应用能够协同地促进骨髓粒－单系造血祖细胞的体外增殖，从而为联合应用造血生长因子来扩增少量骨髓，外周血或脐血中造血干／祖细胞进行移植治疗白血病或实体瘤提供了实验依据。
OBJECTIVE: To study the regulatory effect of different hematopoietic growth factors on the proliferation of bone marrow mononuclear hematopoietic progenitor cells in vitro. Methods: The growth of CFU-GM colonies was detected by methylcellulose in vitro semi-solid culture method. Results: The combination of hematopoietic growth factors could not only increase the number of CFU-GM colonies and promote the formation of CFU-GM colonies in vitro, but also significantly increase the volume of CFU-GM colonies. The proportion of large CFU- The combined application group increased significantly, of which IL-3 + IL-6 + G-CSF + GM-CSF combined application group strongest. At the same time, the results also show that the combination of hematopoietic growth factors can accelerate the formation of CFU-GM colonies. Conclusion: The combined application of hematopoietic growth factor can synergistically promote the proliferation of myeloid granulocyte-monocyte-derived hematopoietic progenitor cells in vitro, so as to expand the hematopoietic stem / progenitor cells in a small amount of bone marrow, peripheral blood or umbilical cord blood in combination with hematopoietic growth factor Treatment of leukemia or solid tumors provide experimental evidence.