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AIM:There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative stress.We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.METHODS:Twenty-four rats divided into three groups werefed with ethanol(6 g/kg/day,Group 1),ethanol and n-acetylcysteine(1g/kg,Group 2),or isocaloric dextrose(control group,Group 3)for 4 weeks.Then animals weresacrificed under ether anesthesia,intracardiac blood andliver tissues were obtained.Measurements were performedboth in serum and in homogenized liver tissues.Malondialdehyde(MDA)level was measured by TBARSmethod.Glutathione peroxidase(GSH-Px)and superoxidedismutase(SOD)levels were studied by commercial kits.Kruskal-Wallis test was used for statistical analysis.RESULTS:ALT and AST in Group 1(154 U/L and 302 U/L,respectively)were higher than those in Group 2(94 U/L and155 U/L)and Group 3(99 U/L and 168 U/L)(P=0.001 forboth).Serum and tissue levels of MDA in Group 1(1.84 nmol/mL and 96 nmol/100 mg-protein)were higher than Group 2(0.91 nmol/mL and 64 nmol/100 mg-protein)and Group 3(0.94 nmol/mL and 49 nmol/100 mg-protein)(P<0.001 forboth).On the other hand,serum GSH-Px level in Group 1(8.21 U/g-Hb)was lower than Group 2(16 U/g-Hb)andGroup 3(16 U/g-Hb)(P<0.001).Serum and liver tissue levelsof SOD in Group 1(11 U/mL and 26 U/100 mg-protein)were lower than Group 2(18 U/mL and 60 U/100 mg-protein)and Group 3(20 U/mL and 60 U/100 mg-protein)(P<0.001for both).CONCLUSION:This study demonstrated that ethanol-induced liver damage is associated with oxidative stress,and co-administration of n-acetylcysteine attenuates thisdamage effectively in rat model.
AIM: There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative stress. We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol. METHODS: Twenty-four rats divided into three groups werefed with ethanol (6 g / kg / day, Group 1), ethanol and n-acetylcysteine (1 g / kg, Group 2), or isocaloric dextrose (control group, Group 3) for 4 weeks. Animals weresacrificed under ether anesthesia, intracardiac Blood andliver tissues were obtained. Measurements were performed in serum and in homogenized liver tissues. Malondialdehyde (MDA) level was measured by TBARSmethod.Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis. RESULTS: ALT and AST in Group 1 (154 U / L and 302 U / L, respectively) were higher than those in Group 2 (94 U / L and 155 U / L) and Group 3 / L and 168 U / L) (P = 0.001 forboth) .Serum and tissue levels of MDA in Group 1 (1.84 nmol / mL and 96 nmol / 100 mg-protein) were higher than Group 2 (0.91 nmol / mL and 64 nmol / 100 mg-protein) (p <0.001 forboth) .On the other hand, serum GSH-Px level in Group 1 (8.21 U / g-Hb) was lower than Group 2 (16 U / g- (P <0.001) .Serum and liver tissue levels of SOD in Group 1 (11 U / mL and 26 U / 100 mg-protein) were lower than Group 2 (18 U / mL and 60 U / 100 mg- protein and Group 3 (20 U / mL and 60 U / 100 mg-protein) (P <0.001 for both) .CONCLUSION: This study demonstrates that ethanol-induced liver damage is associated with oxidative stress, and co-administration of n -acetylcysteine attenuates thisdamage effectively in rat model.