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报道两种长度的ＨＣＶ核心基因片段在Ｅ．ｃｏｌｉ中的表达结果。从ＨＣＶ感染者的血清扩增出核心区基因，利用表达质粒ｐＱＥ－３０，构建了两种重组质粒，其插入片段分别为ＨＣＶ的全长核心区基因（ｃ５７３）和“截断型”基因（ｃ３７５）。经ＩＰＴＧ诱导，含两种质粒的菌株都表达了目的蛋白，ｃ５７３表达的蛋白分子量为２２ｋＤ，表达量占菌体蛋白的８．７％；ｃ３７５表达的蛋白分子量为１９ｋＤ，表达量占菌体蛋白的３９．９％。经Ｎｉ－ＮＴＡ金属螯合层析纯化，获得了高纯度的目的蛋白。这两种蛋白均可特异地与丙型肝炎病人血清发生反应，表明具有良好的抗原活性
Two lengths of HCV core gene fragment are reported in E. coli. Expression in E. coli. The core region genes were amplified from the sera of HCV-infected patients. Two recombinant plasmids were constructed by using the expression plasmid pQE-30. The inserts contained the full-length core region gene (c573) and the truncated gene (c375 ). After IPTG induction, the two plasmids expressed the target protein. The protein expressed by c573 had a molecular weight of 22kD and accounted for 8.7% of the total bacterial protein. The protein expressed by c375 was 19kD, Of the 39.9%. Purified by Ni-NTA metal chelate chromatography to obtain a high purity of the target protein. Both of these proteins can specifically react with hepatitis C patient serum, indicating that they have good antigenic activity