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目的:本实验以前期较成熟湿阻中焦证动物模型为研究平台,研究湿阻中焦证动物模型胃肠组织AQP1的分布和含量以及平胃散的干预,揭示湿阻中焦证动物模型水通道蛋白病理特征性表达分布谱。从蛋白水平揭示湿阻中焦证在水液代谢失调病机方面以及平胃散治疗湿阻中焦证的科学内涵;搭建中药复方水通道调节剂的研究平台。方法:模拟外湿过盛,困阻脾胃、饮食不节,湿从内生、情志不遂,气机受阻,水湿不运三大致湿病因,建立湿阻中焦证模型。用免疫组化技术测定胃肠组织AQP1的分布,用酶联免疫法(ELISA)测定胃肠组织AQP1的含量。结果:湿阻中焦证AQP1在胃贲门黏膜下组织和胃体中间黏膜表达减少,AQP1在胃贲门和小肠中段黏膜表达增加。平胃散增强湿阻中焦证胃贲门黏膜下组织、胃体中间黏膜和小肠中段黏膜AQP1的表达,抑制湿阻中焦证胃贲门黏膜AQP1的表达。结论:AQP1在湿阻中焦证胃肠特征性表达分布谱可能是湿阻中焦证湿邪困阻脾胃,阻遏气机,散输水津失调所表现证候的分子基础之一。平胃散对湿阻中焦证胃肠AQP1表达的干预可能是平胃散燥湿运脾、行气导滞、散中焦之湿阻治疗湿阻中焦证的分子机理之一。平胃散增强正常大鼠胃肠AQP1的表达,可能是平胃散苦燥虽可祛湿但也可伤津液的分子机理之一。平胃散治疗湿阻中焦证的疾病临床疗效确切,但也不能乱服多服。
OBJECTIVE: To investigate the distribution and content of AQP1 in gastrointestinal tissues and the effect of Pingwei Powder in the animal model of wet-resistance mid-focus on the animal model of mature dampness resistance in this experiment. Channel protein pathology characteristic expression profile. From the protein level to reveal the wet resistance in the pathogenesis of focal disturbance in the water and fluid disorders as well as the scientific connotation of Pingwei San in the treatment of dampness; build a research platform for traditional Chinese medicine compound water channel regulator. Methods: To simulate excessive wetness outside the body, obstruction of the spleen and stomach, diet section, wet from the endogenous, emotional failure, the gas machine blocked, wet not wet three major causes of dampness, the establishment of wet resistance in coke card model. The distribution of AQP1 in gastrointestinal tissues was detected by immunohistochemistry and the content of AQP1 in gastrointestinal tissues was determined by enzyme-linked immunosorbent assay (ELISA). Results: The expression of AQP1 in mucous membrane of gastric cardia submucosa tissue and corpus striatum decreased in wet resistance medium, and the expression of AQP1 in mucosa of gastric cardia and middle small intestine increased. Pingweishen enhances the expression of AQP1 in gastric mucosa of gastric cardia, middle gastric mucosa and middle small intestine, and inhibits the expression of AQP1 in gastric cardia mucosa in dampness resistance. Conclusion: The distribution of AQP1 expression in the gastrointestinal tract may be one of the molecular bases of dampness obstructing the spleen and stomach, damaging the qi machine and dispersing dyspepsia. Pingwei San on Wet Resistance Zhongjiao gastrointestinal AQP1 expression intervention may be flat stomach scattered wet spleen, qi stagnation, scattered in the focus of wet resistance in the treatment of wet resistance in the focal mechanism of one of the molecular mechanisms. Pingweisan enhances the expression of AQP1 in normal rats, which may be one of the molecular mechanisms that can relieve dampness when relieving dryness. Pingwei powder treatment of wet resistance in the coke card disease clinical curative effect is exact, but not too much service.