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目的对经cytb基因序列分析证实的青南高原棘球绦虫新种—石渠棘球绦虫进行病原形态学观察,并与其他棘球属绦虫进行形态特征对比。方法收集藏狐肠道石渠棘球绦虫成虫,洋红染色后镜下观察其形态特征。高原鼠兔石渠棘球蚴病理组织用石蜡包埋切片PAS染色后作病理特征检验。成虫及脏器病理组织分别做cytb基因序列分析。结果青南高原发现的石渠棘球绦虫成虫虫体短小(1.2～1.8mm),典型发育的成虫头节、成节和孕节组成,节片数少;顶突钩易脱落,大钩长20.0～24.0μm,小钩长16.0～18.0μm;成虫虫体大小也是目前已知的棘球绦虫中最小的,头钩数18～36枚;生殖孔位置独特,开口于体侧成节前1/3～1/4处,孕节子宫中虫卵数少,孕节子宫不分支,呈袋状。石渠棘球蚴镜下特征性病理变化为:石渠棘球蚴为单房型,直径约10mm。囊内含有育囊,牢固附着在生发层上,内含大量原头节;未见子囊,囊的角质层较厚。主要病理变化为慢性增生性炎症。结论石渠棘球绦虫作为棘球属绦虫的新种在青南高原野生动物中被发现,扩大了对棘球属病原的研究范围。青南高原存在石渠棘球绦虫、多房棘球绦虫、细粒棘球绦虫3种棘球属绦虫;对青南高原石渠棘球绦虫进行的形态学观察,为研究该虫种形态特征、宿主生态分布类型,不同宿主间的循环机制及空间流行病学意义等奠定了基础。
Objective To investigate the pathogen morphology of Echinococcus granulosus, a new species of Echinococcus granulosus confirmed by cytb gene sequence analysis, and to compare with other Echinococcus species. Methods The adult gut spidiocarcinoma collected from Tibetan fox was stained by magenta and observed morphologically. The histopathology of echinococcus granulosus in the plateau rats was examined by PAS staining with paraffin embedded sections. Adult and organ pathology were cytb gene sequence analysis. Results The adults of Schistosoma japonicum found in the Qingnan Plateau were short (1.2 ~ 1.8mm), typical head and adult section, ~ 24.0μm, and the length of the small hook is 16.0 ~ 18.0μm. The size of adult worms is also the smallest among the known echinococcosis species with 18 ~ 36 hooks. The reproductive foramen is uniquely located at 1 / 3 to 1/4 Department, pregnant women, the number of small uterus eggs, uterus pregnancy branch is not branched, was bag. The characteristic pathological changes of the echinococcus granulosum under the microscope include: Echinococcus granulosus is a single-chamber type with a diameter of about 10mm. Capsule contains cysts, firmly attached to the germinal layer, containing a large number of the original head; no ascus, the stratum corneum thicker. The main pathological changes of chronic proliferative inflammation. Conclusion The new species of Echinococcus granulosus, as a new species of Echinococcus, was found in wild animals in the Qingnan Plateau, expanding the scope of the study on echinococcosis. There were three species of Echinococcus granulosus, Echinococcus multilocularis and Echinococcus granulosus in Qingnan Plateau. Morphological observation of Echinococcus granulosus in Qingnan Plateau was conducted to investigate the morphological characteristics of the species, The type of ecological distribution, the circulation mechanism between different hosts and the significance of space epidemiology.