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NAG(N-乙酰-β-氨基糖葡萄糖苷酶)是溶酶体中的一种蛋白水解酶,参与蛋白多糖的分解代谢。因此,血清中NAG活力的升高有助于肝纤维化的诊断。我们对正常人,慢性肝炎及肝硬化患者血清NAG活力作了研究,现报告如下。一.研究对象1.正常献血员100名,2.慢性活动性肝炎39例(1984年郑州会议标准);3.肝硬化病人29例(经肝病理证实20例。钡餐造影示食管静脉曲张及B超示硬化共9例);4.慢性胆囊炎胆石症8例。二.材料和方法血清NAG活力测定采用改良的Grenessner方法。酶底物为N-乙酰-β-葡萄糖胺,分子量342(北京工业制药研究所制)。
NAG (N-acetyl-β-aminoglycosidase) is a proteolytic enzyme in lysosomes that is involved in the catabolism of proteoglycans. Therefore, increased serum NAG activity contributes to the diagnosis of liver fibrosis. We have normal, chronic hepatitis and cirrhosis serum NAG activity were studied, are as follows. A study of 100 normal blood donors, 2 chronic active hepatitis in 39 cases (1984 Zhengzhou meeting standard), 3 liver cirrhosis in 29 cases (liver pathology confirmed 20 cases of barium meal showed esophageal varices and B ultrasound showed a total of 9 cases); 4 chronic cholecystitis cholelithiasis in 8 cases. Materials and methods Serum NAG activity was measured using a modified Grenessner method. The enzyme substrate was N-acetyl-β-glucosamine, and the molecular weight was 342 (manufactured by Beijing Institute of Pharmaceutical Industry).